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Chloroplast and mitochondrial microsatellites for (Fabaceae) and cross-amplification in related species. | LitMetric

Chloroplast and mitochondrial microsatellites for (Fabaceae) and cross-amplification in related species.

Appl Plant Sci

Key Laboratory of Tropical Agro-Environment, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, Guangdong, People's Republic of China.

Published: May 2017

Premise Of The Study: Chloroplast and mitochondrial microsatellites were identified to study the population genetics of (Fabaceae).

Methods And Results: Based on publicly available plastid genome sequence data of , 42 primer pairs were developed, of which 17 displayed polymorphisms across 89 individuals from four populations. For chloroplast loci, two to six alleles were recovered and the unbiased haploid diversity per locus ranged from 0.391 to 0.857. For mitochondrial loci, two to four alleles were recovered and the unbiased haploid diversity ranged from 0.264 to 0.740. Sixteen of the 17 screened markers could be successfully amplified in the related species .

Conclusions: The 17 microsatellite markers developed here exhibited variation in and 16 presented transferability in the related species , suggesting that these markers will be valuable for genetic studies across and its related species.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435409PMC
http://dx.doi.org/10.3732/apps.1700034DOI Listing

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