Introduction: The role of complement, especially through the C5a fragment, is well-known for the initiation of inflammation. Its involvement in regeneration has been shown more recently by the recruitment of mesenchymal stem cells. C5a can be produced locally by the pulp fibroblasts in response to injury or infection. This work aims to investigate the effect of different pulp capping biomaterials on complement activation and its possible influence on inflammatory and pulp stem cell recruitment.
Methods: Conditioned media were prepared from 3 pulp capping biomaterials: Biodentine (Septodont, Saint-Maur-des-Fosses, France), TheraCal (BISCO, Lançon De Provence, France), and Xeno III (Dentsply Sirona, Versaille, France). Injured pulp fibroblasts were cultured with these conditioned media to analyze C5a secretion using an enzyme-linked immunosorbent assay. Dental pulp stem cells (DPSCs) were isolated from human third molar explants by magnetic cell sorting with STRO-1 antibodies. The expression of C5a receptor on DPSCs and inflammatory (THP-1) cells was investigated by immunofluorescence. The migration of both DPSCs and THP-1 cells was studied in Boyden chambers.
Results: Pulp fibroblast production of C5a significantly increased when the cells were incubated with TheraCal- and Xeno III-conditioned media. The recruitment of cells involved in inflammation (THP-1 cells) was significantly reduced by Biodentine- and TheraCal-conditioned media, whereas the migration of DPSCs was reduced with TheraCal- and Xeno III-conditioned media but not with that of Biodentine. The involvement of C5a in cell recruitment is demonstrated with a C5a receptor-specific antagonist (W54011).
Conclusions: After pulp injury, the pulp capping material affects complement activation and the balance between inflammation and regeneration through a differential recruitment of DPSCs or inflammatory cells.
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http://dx.doi.org/10.1016/j.joen.2017.02.016 | DOI Listing |
J Indian Soc Pedod Prev Dent
October 2024
Department of Paediatric and Preventive Dentistry, T.P.C.T's Terna Dental College, Navi Mumbai, Maharashtra, India.
Objectives: Comparative evaluation of indirect pulp therapy (IPT) with silver diamine fluoride (SDF), Type VII glass ionomer cement (GIC), and calcium hydroxide (Ca(OH)2) in young permanent molars.
Materials And Methods: This was randomized controlled trial, in which 45 children with 60 young permanent first molars were allocated as; Group A: IPT with SDF, Group B: Type VII GIC, and Group C: Ca(OH)2. Clinical and radiographic evaluation and comparison was done at baseline, 3, 6, 12 months.
Br Dent J
January 2025
Key Laboratory of Shaanxi Province for Craniofacial Precision Medical Research, College of Stomatology, Xi´an Jiaotong University, Xi´an, P.R. China; Department of Paediatric Dentistry, Affiliated Stomatology Hospital of Xi´an Jiaotong University, Xi´an, P.R. China.
Background Indirect pulp capping (IDPC) is a preferred treatment for pulp preservation in primary teeth. However, the survival rate of IDPC in primary teeth and impact factors is still equivocal.Aims To evaluate the survival rate of IDPC in primary teeth with a deep carious lesion approximating the pulp but without irreversible pulpitis or periapical disease.
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January 2025
Oral Radiology, Faculty of Dentistry, Cairo University, Giza, Egypt.
Aim: Clinical and radiographic evaluation of SDF versus MTA as indirect pulp capping agents in deeply carious first permanent molars.
Methodology: This study was conducted on (30) first permanent molars indicated for indirect pulp capping (IPC) randomly allocated to either SDF or MTA groups (n = 15). The molars were finally restored with glass hybrid glass ionomer restoration.
Int J Clin Pediatr Dent
November 2024
Department of Pediatric and Preventive Dentistry, Shree Guru Gobind Singh Tricentenary Dental College, Hospital and Research Institute, Gurugram, Haryana, India.
Aim: The present case-control study was planned to assess the comparative efficacy of resin-modified calcium silicate, resin-modified glass ionomer, and Dycal as pulp capping agents in indirect pulp therapy for deeply carious young permanent molars.
Materials And Methods: Thirty deeply carious young posterior teeth were treated by indirect pulp therapy. During the treatment, the cavity floor was lined with TheraCal or resin-modified glass ionomer cement (RMGIC) in the study group and with Dycal (control group) followed by GC IX and composite restoration.
Adv Mater
January 2025
Department of Chemistry, University College London, London, WC1E 7JE, UK.
Long-standing challenges including notorious side reactions at the Zn anode, low Zn anode utilization, and rapid cathode degradation at low current densities hinder the advancement of aqueous zinc-ion batteries (AZIBs). Inspired by the critical role of capping agents in nanomaterials synthesis and bulk crystal growth, a series of capping agents are employed to demonstrate their applicability in AZIBs. Here, it is shown that the preferential adsorption of capping agents on different Zn crystal planes, coordination between capping agents and Zn ions, and interactions with metal oxide cathodes enable preferred Zn (002) deposition, water-deficient Zn ion solvation structure, and a dynamic cathode-electrolyte interface.
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