Objectives: This study aims to investigate the small intestinal submucosal (SIS) surface after heparinization with the hypothermia plasma technique, to improve the blood compatibility of SIS, and to explore the possibility of construction of small-caliber vascular grafts with modified SIS scaffolds in vivo.
Methods: SIS films prepared from jejunums of pigs were processed for surface treatment at different time periods with the argon plasma initiation technique under vacuum, and were then immediately immersed in 4% (m/v) heparin sodium solution for 24-h heparinization. The surface morphologies of heparinized SIS were observed under a scanning electron microscope (SEM). The antithrombogenicity of the modified SIS films was tested by measuring the water contact angle, blood coagulation time, activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT), and observation of platelet adherence by SEM. Heparinized SIS films were sewn into a small caliber (3-mm) tube and implanted into the defect of a canine femur by anastomosis as a vascular graft. The efficiency of the SIS graft was evaluated according to the patency for the circulation of blood with Doppler color ultrasonography and hematoxylin-eosin staining.
Results: Heparinized SIS showed a significantly different surface morphology compared with that of untreated SIS. The SIS surface resembles wrinkled film, but the heparinized SIS surface is uniformly coated with microdots, and appears to have a layer of heparin adhesion.
Conclusion: Heparin was attached to the SIS surface after hypothermia plasma treatment. Hydrophilicity and antithrombogenicity of heparinized SIS were clearly increased. The heparinized SIS vascular graft showed great potential for replacement of defective small-caliber vessels.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3233/BME-171668 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Klotho functionalization on vascular graft for improved patency and endothelialization.
Biomater Adv
February 2022
Department of Tissue Engineering, School of Intelligent Medicine, China Medical University, Shenyang 110122, China. Electronic address:
The Klotho (KL) gene is related to aging. In this study, SKL (secreted KL) and heparin were cross-linked to the acellular small intestinal submucosa (SIS). Based on this, tissue-engineered bioactive small blood vessels were constructed.
View Article and Find Full Text PDFTranshepatic Access: Alternative Approach for Right Heart Catheterization and Pulmonary Angiography.
Cardiovasc Revasc Med
July 2022
Section of Interventional Cardiology, MedStar Washington Hospital Center, Washington, DC, United States of America.
Peripheral vascular disease, chronic large-bore venous access, and small body habitus can limit traditional venous access methods for right heart catheterization. We present a patient with chronic renal failure, small body habitus due to developmental delay, and multiple central vein stenoses due to chronic indwelling catheters and heparin-induced thrombocytopenia, who required right heart catheterization for workup of possible intracardiac shunt, pulmonary hypertension, and pulmonary embolism. We performed right heart catheterization and pulmonary angiography via transhepatic approach.
View Article and Find Full Text PDFPrevention of bleeding after percutaneous biopsy with a small intestinal submucosa hemostatic plug.
Acta Biomater
January 2022
Department of Biomedical Engineering, Universidad de los Andes, Colombia; Research Department Fundación Cardioinfantil - Instituto de Cardiología, Colombia. Electronic address:
Percutaneous biopsies (PBs) are the gold standard diagnostic procedures indicated for renal and hepatic disorders. Nevertheless, they can cause hemorrhages and are contraindicated for coagulopathic patients. In this study we designed, fabricated, and evaluated a small intestinal submucosa (SIS) plug to reduce, and potentially cease, bleeding to decrease death risk after percutaneous hepatic and renal biopsies in healthy and coagulopathic in vivo models.
View Article and Find Full Text PDFCell-free vascular grafts that grow with the host.
Adv Funct Mater
November 2020
Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Amherst, NY 14260-4200, USA.
Cell-free small diameter vascular grafts, based on small intestinal submucosa (SIS) functionalized with heparin and vascular endothelial growth factor (VEGF) manufactured and implanted successfully into the arterial system of neonatal lambs, where they remained patent and grew in size with the host to a similar extent and with similar rate as native arteries. Acellular tissue engineered vessels (A-TEV) integrated seamlessly into the native vasculature and developed confluent, functional endothelium that afforded patency. The medial layer was infiltrated by smooth muscle cells, showed no signs of calcification and developed contractile function.
View Article and Find Full Text PDFCreation of Bony Microenvironment with Extracellular Matrix Doped-Bioactive Ceramics to Enhance Osteoblast Behavior and Delivery of Aspartic Acid-Modified BMP-2 Peptides.
Int J Nanomedicine
November 2020
Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People's Republic of China.
Introduction: Decellularized matrix from porcine small intestinal submucosa (SIS) endows scaffolds with an ECM-like surface, which enhances stem cell self-renewal, proliferation, and differentiation. Mesoporous bioactive glass (MBG) is extensively recognized as an excellent bio-ceramic for fabricating bone grafts.
Materials And Methods: In the current study, SIS was doped on an MBG scaffold (MBG/SIS) using polyurethane foam templating and polydopamine chemistry method.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!