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Chemical Structure of the Lipid A component of Pseudomonas sp. strain PAMC 28618 from Thawing Permafrost in Relation to Pathogenicity. | LitMetric

AI Article Synopsis

  • Climate change is leading to the thawing of permafrost, which poses risks due to the release of previously dormant microbes that can cause diseases.
  • Researchers used MALDI-based mass spectrometry to analyze the lipid A structure of a Pseudomonas species isolated from thawing arctic permafrost, revealing detailed chemical characteristics.
  • The findings indicated that the lipid A from this strain resembles that of another harmful Pseudomonas, suggesting potential pathogenic similarities and providing a framework for assessing the dangers of bacteria emerging from permafrost.

Article Abstract

Climate change causes permafrost thawing, and we are confronted with the unpredictable risk of newly discovered permafrost microbes that have disease-causing capabilities. Here, we first characterized the detailed chemical structure of the lipid A moiety from a Pseudomonas species that was isolated from thawing arctic permafrost using MALDI-based mass spectrometric approaches (i.e., MALDI-TOF MS and MALDI-QIT-TOF MS). The MALDI multi-stage mass spectrometry (MS) analysis of lipid A extracted from the Pseudomonas sp. strain PAMC 28618 demonstrated that the hexaacyl lipid A ([M-H] at m/z 1616.5) contains a glucosamine (GlcN) disaccharide backbone, two phosphates, four main acyl chains and two branched acyl chains. Moreover, the lipid A molecule-based structural activity relationship with other terrestrial Gram-negative bacteria indicated that strain PAMC 28618 has an identical lipid A structure with the mesophilic Pseudomonas cichorii which can cause rot disease in endive (Cichorium endivia) and that their bacterial toxicities were equivalent. Therefore, the overall lipid A validation process provides a general strategy for characterizing bacteria that have been isolated from arctic permafrost and analyzing their respective pathogenicities.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5438365PMC
http://dx.doi.org/10.1038/s41598-017-02145-wDOI Listing

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