AI Article Synopsis
- The amino acid 4-fluorohistidine has a lower pK than histidine, giving proteins that use it unique properties.
- Researchers have optimized a method to synthesize 4-fluorohistidine and confirmed it effectively replaces histidine in translation processes.
- Peptides with 6×-fluorohistidine tags can be specifically isolated from mixtures containing histidine-tagged proteins using nickel resin.
Article Abstract
Due to the lowered pK of 4-fluorohistidine relative to histidine, peptides and proteins containing this amino acid are potentially endowed with novel properties. We report here the optimized synthesis of 4-fluorohistidine and show that it can efficiently replace histidine in in vitro translation reactions. Moreover, peptides containing 6×-fluorohistidine tags are able to be selectively captured and eluted from nickel resin in the presence of his-tagged protein mixtures.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6010304 | PMC |
| http://dx.doi.org/10.1039/c7ob00844a | DOI Listing |
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