An enzyme-labeled complement fixation (ELISA-CF) test for the direct and quantitative determination of complement fixing (CF) antibodies has been developed. This paper described the introduction of the ELISA-CF test that used peroxidase-labeled Clq component of complement to detect CF antibodies which had reacted with herpes simplex virus (HSV), as a virus model. Equal volumes of heat-inactivated serum and the peroxidase-labeled Clq (P*-Clq) were simultaneously added to wells of microplates which had been coated with HSV CF antigen or with cell control antigen. The enzymatic activities of P*-Clq bound to the immune complex were determined photometrically. The ELISA-CF test allows processing of serum specimens in a 3-hr operation, with procedural simplicity and increased specificity and sensitivity compared with the conventional CF test.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1348-0421.1988.tb01480.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Serodiagnosis of Babesia equi infection--a comparison of Dot-ELISA, complement fixation test and capillary tube agglutination test.
Vet Parasitol
May 1997
AICRP on Blood Protista, College of Veterinary Sciences, CCS Haryana Agricultural University, Hisar, Haryana, India.
The present study aimed to develop Dot-ELISA, complement fixation test (CFT) and capillary tube agglutination test (CAT) for serodiagnosis of Babesia equi infection and to compare their sensitivity with each other. For this study, sequential serum samples were collected from four donkeys experimentally infected with B. equi up to 90 days post infection (P.
View Article and Find Full Text PDFComparative performance of the enzyme-linked immunosorbent assay (ELISA) and conventional assays in the diagnosis of bovine brucellosis in Argentina.
Vet Immunol Immunopathol
July 1995
Instituto de Bacteriologia, Instituto Nacional de Tecnologia Agropecuarias, Moron Provincia de Buenos Aires, Argentina.
The FAO/IAEA enzyme-linked immunosorbent assay (ELISA) kit for the diagnosis of bovine brucellosis was compared in Argentina with two screening tests, the Rose Bengal (RB) and the buffered plate antigen (BPA) agglutination tests and with two confirmatory tests, the 2-mercaptoethanol (2-ME) agglutination and the complement fixation (CF) tests. In the testing of Brucella abortus Strain 19 (S19) vaccinated cattle from Brucella-free dairy herds, the diagnostic specificity estimate of the ELISA (99.7%) was shown to be comparable to the RB (99.
View Article and Find Full Text PDFRapid identification and typing of herpes simplex virus by a new enzyme immunoassay with peroxidase-labeled complement C1q.
Arch Virol
January 1990
Department of Microbiology, School of Hygienic Sciences, Kitasato University, Kanagawa, Japan.
A new enzyme-linked immunoassay system (TC-ELISA) was developed for the rapid, specific detection and typing of herpes simplex virus (HSV) using anti-HSV immune sera and enzyme-labeled complement component C1q (P*-C1q). The method is based on viral antigen produced in HSV-infected cells being detected by simultaneous addition of immune serum and P*-C1q (ELISA-CF). With this assay, it was found that HSV was detected with anti-HSV immune serum and P*-C1q and that HSV type 1 and HSV type 2 could be differentiated with anti-HSV-1 and anti-HSV-2 immune sera and P*-C1q.
View Article and Find Full Text PDFNew complement fixation test with peroxidase-labeled complement Clq for direct and quantitative determination of antibodies to herpes simplex virus.
Microbiol Immunol
March 1989
Department of Microbiology, School of Hygienic Sciences, Kitasato University, Kanagawa.
An enzyme-labeled complement fixation (ELISA-CF) test for the direct and quantitative determination of complement fixing (CF) antibodies has been developed. This paper described the introduction of the ELISA-CF test that used peroxidase-labeled Clq component of complement to detect CF antibodies which had reacted with herpes simplex virus (HSV), as a virus model. Equal volumes of heat-inactivated serum and the peroxidase-labeled Clq (P*-Clq) were simultaneously added to wells of microplates which had been coated with HSV CF antigen or with cell control antigen.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!