AI Article Synopsis
- - The study investigates the role of the Qc-SNARE protein AtBS14b in the brassinosteroid (BR) signaling pathway in Arabidopsis, focusing on its effects on plant morphology and BR responsiveness.
- - Overexpressing AtBS14b led to shorter hypocotyls and petioles, and reduced sensitivity to BR, while AtBS14b mutants showed no significant morphological changes related to BR signaling.
- - AtBS14b was found to directly interact with the membrane steroid binding protein 1 (MSBP1), suggesting its unique function in modulating BR responses in plants, marking it as the first SNARE protein known to interact with MSBP1.
Article Abstract
Background: N-ethyl-maleimide sensitive factor adaptor protein receptor (SNAREs) domain-containing proteins were known as key players in vesicle-associated membrane fusion. Genetic screening has revealed the function of SNAREs in different aspects of plant biology, but the role of many SNAREs are still unknown. In this study, we have characterized the role of Arabidopsis Qc-SNARE protein AtBS14b in brassinosteroids (BRs) signaling pathway.
Results: AtBS14b overexpression (AtBS14b ox) plants exhibited short hypocotyl and petioles lengths as well as insensitivity to exogenously supplied BR, while AtBS14b mutants did not show any visible BR-dependent morphological differences. BR biosynthesis enzyme BR6OX2 expression was slightly lower in AtBS14b ox than in wild type plants. Further BR-mediated repression of BR6OX2, CPD and DWF4 was inhibited in AtBS14b ox plants. AtBS14b-mCherry fusion protein localized in vesicular compartments surrounding plasma membrane in N. benthamiana leaves. In addition, isolation of AtBS14b-interacting BR signaling protein, which localized in plasma membrane, showed that AtBS14b directly interacted with membrane steroid binding protein 1 (MSBP1), but did not interact with BAK1 or BRI1.
Conclusion: These data suggested that Qc-SNARE protein AtBS14b is the first SNARE protein identified that interacts with MSBP1, and the overexpression of AtBS14b modulates BR response in Arabidopsis.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430330 | PMC |
| http://dx.doi.org/10.1186/s40529-014-0055-5 | DOI Listing |
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Overexpression of a SNARE protein AtBS14b alters BR response in Arabidopsis.
Bot Stud
December 2014
Agricultural College, Anhui Agricultural University, Changjiangxi Road 130, Hefei, 230036, Anhui, China.
Article Synopsis
- - The study investigates the role of the Qc-SNARE protein AtBS14b in the brassinosteroid (BR) signaling pathway in Arabidopsis, focusing on its effects on plant morphology and BR responsiveness.
- - Overexpressing AtBS14b led to shorter hypocotyls and petioles, and reduced sensitivity to BR, while AtBS14b mutants showed no significant morphological changes related to BR signaling.
- - AtBS14b was found to directly interact with the membrane steroid binding protein 1 (MSBP1), suggesting its unique function in modulating BR responses in plants, marking it as the first SNARE protein known to interact with MSBP1.
AtBS14a and AtBS14b, two Bet1/Sft1-like SNAREs from Arabidopsis thaliana that complement mutations in the yeast SFT1 gene.
FEBS Lett
July 2001
Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PR China.
SNAREs are membrane-associated proteins that play a central role in vesicle targeting and intra-cellular membrane fusion reactions in eukaryotic cells. Here we describe the identification of AtBS14a and AtBS14b, putative SNAREs from Arabidopsis thaliana that share 60% amino acid sequence identity. Both AtBS14a and BS14b are dosage suppressors of the temperature-sensitive growth defect in sft1-1 cells and over-expression of either AtBS14a or AtBS14b can support the growth of sft1Delta cells but not bet1Delta cells.
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