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Cryo-EM structure of the conjugation H-pilus reveals the cyclic nature of the TrhA pilin.
bioRxiv
December 2024
Rutherford Appleton Laboratory, Research Complex at Harwell, Didcot, Oxfordshire, UK.
Conjugation, the major driver of the spread of antimicrobial resistance genes, relies on a conjugation pilus for DNA transfer. Conjugative pili, such as the F-pilus, are dynamic tubular structures, composed of a polymerized pilin, that mediate the initial donor-recipient interactions, a process known as mating pair formation (MPF). IncH are low-copy-number plasmids, traditionally considered broad host range, which are found in bacteria infecting both humans and animals.
View Article and Find Full Text PDFMicrobe Profile: Typhimurium: the master of the art of adaptation.
Microbiology (Reading)
January 2025
Clinical Infection, Microbiology & Immunology Department, Institute of Infection, Veterinary & Ecological Sciences, University of Liverpool, Liverpool, UK.
Typhimurium is a major serovar that is found globally. It is responsible for outbreaks of self-limiting gastroenteritis that are broadly linked to the industrialization of food production. .
View Article and Find Full Text PDFCustomized design of host-independent T7 expression system (HITES) for a broad host range.
J Biotechnol
February 2025
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address:
Efficient methods and universal DNA elements are eagerly required for the expression of proteins and the production of target chemicals in synthetic biology and metabolic engineering. This paper develops a customized-design approach by utilizing the host-independent T7 expression system (HITES), which facilitates the rational design and rapid construction of T7 expression systems. Firstly, the EL (Upper-limit value of initial enzyme activity) value is discovered to play a pivotal factor in the successful construction of the T7 expression system, different host strains exhibit varying EL values, and this study presents a method to measure the EL values.
View Article and Find Full Text PDFAdjuvant therapy with bacteriophage (phage) cocktails in combination with antibiotics is a therapeutic approach currently considered for treatment of infections with encapsulated, biofilm forming, and multidrug-resistant Klebsiella pneumoniae (Kp). Klebsiella phage are highly selective in targeting a bacterial capsule type. Considering the numerous Kp capsule types and other host restriction factors, phage treatment could be facilitated when generating phages with a broad host range.
View Article and Find Full Text PDFIdentification of plasmids from thermophilic Streptomyces strains and development of a gene cloning system for thermophilic Streptomyces species.
J Ind Microbiol Biotechnol
January 2024
Technology Research Association for Next-Generation Natural Products Chemistry, 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan.
Unlabelled: To develop a host-vector system for use in thermophilic Streptomyces, multi-copy plasmids were screened for thermophilic Streptomyces species using data from public bioresource centers (JCM and NBRC). Of 27 thermophilic Streptomyces strains, 3 harbored plasmids. One plasmid (pSTVU1), derived from S.
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