AI Article Synopsis
- The study aimed to evaluate the impact of Qishen Granule (QSG) on calcium handling in the sarcoplasmic reticulum of heart failure (HF) rats and identify the related molecular mechanisms.
- Researchers created HF models in rats through surgery and a high-fat diet, then divided them into groups for treatment with QSG, metoprolol, or a sham procedure, assessing heart function and calcium dynamics.
- Findings revealed that QSG improved heart function and corrected calcium deficiencies by regulating key proteins involved in calcium cycling, making it a promising alternative treatment for heart failure.
Article Abstract
Objective: To assess the effects of Qishen Granule (, QSG) on sarcoplasmic reticulum (SR) Ca handling in heart failure (HF) model of rats and to explore the underlying molecular mechanisms.
Methods: HF rat models were induced by left anterior descending coronary artery ligation surgery and high-fat diet feeding. Rats were randomly divided into sham (n=10), model (n=10), QSG (n=12, 2.2 g/kg daily) and metoprolol groups (n=12, 10.5 mg/kg daily). The therapeutic effects of QSG were evaluated by echocardiography and blood lipid testing. Intracellular Ca concentration and sarco-endoplasmic reticulum ATPase 2a (SERCA2a) activity were detected by specifific assay kits. Expressions of the critical regulators in SR Ca handling were evaluated by Western blot and real-time quantitative polymerase chain reaction.
Results: HF model of rats developed ventricular remodeling accompanied with calcium overload and defective Ca release-uptake cycling in cardiomyocytes. Treatment with QSG improved contractive function, attenuated ventricular remodeling and reduced the basal intracellular Ca level. QSG prevented defective Ca leak by attenuating hyperphosphorylation of ryanodine receptor 2, inhibiting expression of protein kinase A and up-regulating transcriptional expression of protein phosphatase 1. QSG also restored Ca uptake by up-regulating expression and activity of SERCA2a and promoting phosphorylation of phospholamban.
Conclusion: QSG restored SR Ca cycling in HF rats and served as an ideal alternative drug for treating HF.
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| http://dx.doi.org/10.1007/s11655-017-2809-x | DOI Listing |
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