AI Article Synopsis
- Lipase enzymes are important for various industries, and their production cost is influenced by the composition of culture media, which researchers continually optimize.
- The study used a "one variable at a time" approach to evaluate different carbon and nitrogen sources in the culture medium, along with incubation times, to maximize lipase production.
- Results indicated that the highest lipase activity was achieved using low-cost sources like glucose syrup, fish powder, and olive oil during 24 hours of incubation, potentially lowering production costs for industrial use.
Article Abstract
Background: Lipase enzymes have applications in a wide range of industries. A crucial determining factor of industrial prices of these enzymes is the culture media composition that is constantly under review by researchers. In this work, for maximum lipase production by , culture media compositions were optimized using ″one variable at a time″ strategy.
Methods: For this purpose, the culture medium parameters such as low and high cost carbon and nitrogen sources, substrates and incubation times were evaluated.
Results: Maximum lipase activity was achieved after 24 of incubation with 1.5% of glucose syrup (1600±69.1 ), 1% of fish powder (1238±36.7 ) and olive oil (1407±2.1 ) as low cost carbon and nitrogen sources and substrate, respectively.
Conclusion: Our results show a significant increase in lipase activity with usage of low cost sources; this could help in reducing the media prices for industrial application of lipase enzyme.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5410133 | PMC |
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