AI Article Synopsis
- The overall growth of DNA strands during replication occurs in two directions: leading strands grow continuously in a 5'-to-3' direction, while lagging strands grow in pieces (Okazaki fragments) in a 3'-to-5' direction due to enzyme limitations.
- Discontinuous Replication Mechanism is used to synthesize these lagging strand segments, involving several steps like synthesizing RNA primers, creating Okazaki fragments, removing primers, and filling gaps.
- Key enzymes involved in this process include RNase H, DNA polymerase I for filling in gaps, and DNA ligase to join the Okazaki fragments into a continuous strand.
Article Abstract
At DNA replication forks, the overall growth of the antiparallel two daughter DNA chains appears to occur 5'-to-3' direction in the leading-strand and 3'-to-5' direction in the lagging-strand using enzyme system only able to elongate 5'-to-3' direction, and I describe in this review how we have analyzed and proved the lagging strand multistep synthesis reactions, called Discontinuous Replication Mechanism, which involve short RNA primer synthesis, primer-dependent short DNA chains (Okazaki fragments) synthesis, primer removal from the Okazaki fragments and gap filling between Okazaki fragments by RNase H and DNA polymerase I, and long lagging strand formation by joining between Okazaki fragments with DNA ligase.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5489436 | PMC |
| http://dx.doi.org/10.2183/pjab.93.020 | DOI Listing |
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