AI Article Synopsis
- The CRNDE gene, a long noncoding RNA, is found to be increased in gastric cancer and may play a role in regulating cell growth.
- Research methods included assessing expression levels through qRT-PCR and examining cell viability with MTT assays, revealing that higher CRNDE levels correlated with increased growth and colony formation of gastric cancer cells.
- The study highlights CRNDE's interaction with the miR-145 and its target E2F3, suggesting that CRNDE could be a potential target for gastric cancer therapies.
Article Abstract
Background/aims: The colorectal neoplasia differentially expressed (CRNDE) gene is a long noncoding RNA (lncRNAs) that is upregulated in colorectal cancer and glioma. Here, we investigated the regulatory function of CRNDE in gastric cancer (GC).
Methods: CRNDE and miR-145 expression were assayed by qRT-PCR, and E2F3 protein expression was measured by western blotting. A luciferase reporter assay was used to detect the direct regulation of miR-145 by CRNDE. Cell viability and colony formation of human GC cells were detected using MTT and colony formation assay, respectively.
Results: CRNDE was highly expressed in GC cell lines and tissues; overexpression of CRNDE increased GC cell viability and promoted colony formation. Knockdown of CRNDE did not result in loss of expression-related effects on cell proliferation and colony formation. Further investigation revealed that the miR-145 target gene E2F3 was strongly expressed following CRNDE competitive molecular sponging of miR-145.
Conclusion: CRNDE acted as a growth-promoting lncRNA in GC and maybe a potential target of GC treatment.
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| http://dx.doi.org/10.1159/000477107 | DOI Listing |
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