Aims: Transplantation of a tissue engineered cardiac pacemaker (TECP) may represent a novel therapy for cardiac sinus node dysfunction. We previously reported that cardiac progenitor cells (CPCs) derived from embryonic heart tubes could differentiate into cardiac pacemaking cells after endothelin-1 treatment. We aimed to examine the feasibility of TECP fabricated from CPCs-derived pacemaking cells and vascularization of TECP fabricated from CPCs-derived pacemaking cells and endothelial progenitor cells (EPCs) in vitro and in vivo implantation.
Main Methods: TECP created using CPCs-derived pacemaking cells and vTECP created by mixing CPCs and EPCs in vitro were implanted into rat hearts. Sinus node damaged was induced by formaldehyde insult.
Key Results: Spontaneous beating tissues, namely TECP, were obtained after seeding CPCs-derived pacemaking cells into Matrigel. ECG and epicardial multielectrode array (MEA) measurements confirmed implanted TECP have electrical activity. TECP implantation promoted individual survival in sinus node damage models (15/22 animals lived versus 0/17 control). vTECP fabricated by mixing the both EPCs and CPCs-derived pacemaking cells with Matrigel in equal proportions optimally formed pre-vascularization in vitro. The implantation of vTECP enhanced electrical activity in vivo, which may correlate with increased vascularization. PI3K-Akt-VEGF/VEGFR signaling was involved with vascular ingrowth in vTECP.
Significance: Our data supports the therapeutic potential of TECP fabricated with the CPCs-derived pacemaking cells for sinus node dysfunction. Vascularization by the addition of EPCs is an important factor to sustain viability of the TECP in vivo.
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http://dx.doi.org/10.1016/j.lfs.2017.05.007 | DOI Listing |
Phys Rev E
November 2024
Laboratory of Dynamics in Biological Systems, Department of Cellular and Molecular Medicine, KU Leuven, Herestraat 49, 3000 Leuven, Belgium.
We explore a biomimetic model that simulates a cell, with the internal cytoplasm represented by a two-dimensional circular domain and the external cortex by a surrounding ring, both modeled using FitzHugh-Nagumo systems. The external ring is dynamically influenced by a pacemaker-driven wave originating from the internal domain, leading to the emergence of three distinct dynamical states based on the varying strengths of coupling. The range of dynamics observed includes phase patterning, the propagation of phase waves, and interactions between traveling and phase waves.
View Article and Find Full Text PDFJ Insect Physiol
December 2024
Department of Cell Biology and Imaging, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, Krakow, Poland. Electronic address:
The visual system is a sensory system which is sensitive to light and detects photic stimuli. It plays many important functions, such as vision, circadian clock entrainment and regulation of sleep-wake behavior. The interconnection between the visual system and clock network is precisely regulated.
View Article and Find Full Text PDFElife
December 2024
Department of Pharmacology, Hebei Medical University, Shijiazhuang, China.
Nat Commun
December 2024
Department of Neuroscience, Karolinska Institutet, 171 77, Stockholm, Sweden.
Int J Med Sci
December 2024
Department of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University, Yangsan 50612, Republic of Korea.
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