Background: DNA barcoding is a widely used tool that enables rapid and accurate identification of species based on standardized DNA regions.
Materials And Methods: In this study, potential DNA barcodes, namely three plastid regions (L, H-A and K) and one nuclear ribosomal internal transcribed spacer (ITS) were adopted for species identification of original plants of Fritillariae Cirrhosae Bulbus.
Results: The L and H-A regions showed better success rate of PCR amplification and DNA sequencing, as well as superior discriminatory ability. On the contrary, ITS region did not possess effective genetic variation and K was faced with low success rate of sequencing. Combination of multi-loci sequences could improve identification ability of DNA barcoding. The H-A + rbcL could discriminate 25% - 100% species based on the Blast, Tree-Building and Distance methods.
Conclusion: The potential DNA barcodes could not completely solving species identification of botanic origins of Fritillariae Cirrhosae Bulbus. In future, we should pay more attention to super-barcoding or specific barcode that enhance ability to discriminate the closely related plants.
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| http://dx.doi.org/10.21010/ajtcam.v13i6.12 | DOI Listing |
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