Fourier Transform Infrared Spectroscopy as a Tool in Analysis of Proteus mirabilis Endotoxins.

Methods Mol Biol

Department of Microbiology, Jan Kochanowski University, Świętokrzyska 15, PL-25-406, Kielce, Poland.

Published: February 2018

Fourier transform infrared spectroscopy (FT-IR) was used to scan whole bacterial cells as well as lipopolysaccharides (LPSs, endotoxins) isolated from them. Proteus mirabilis cells, with chemically defined LPSs, served as a model for the ATR FT-IR method. The paper focuses on three steps of infrared spectroscopy: (1) sample preparation, (2) IR scanning, and (3) multivariate analysis of IR data (principal component analysis, PCA).

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http://dx.doi.org/10.1007/978-1-4939-6958-6_11DOI Listing

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