Schizochytrium is a microalgae-like fungus and is widely used for producing docosahexaenoic acid (DHA). It is also a promising source of squalene and carotenoids. However, few fermentation strategies are available in enhancing squalene and carotenoid content in Schizochytrium. This study showed that butanol addition had multiple effects on Schizochytrium limacinum B4D1. First, butanol addition altered the lipid content of cells. Second, 6g/L of butanol decreased the proportion of DHA by nearly 40%. Third, the squalene content increased 31-fold in the presence of 6g/L butanol. Finally, cells accumulated more carotenoids upon butanol addition. Specifically, when cells were treated with 8g/L butanol, the astaxanthin content increased to 245 times than that of the untreated control. These results are helpful for the commercial exploitation of Schizochytrium in producing squalene and carotenoids.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.enzmictec.2017.03.007 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Pre-exposure of Lactobacillus acidophilus to stress conditions impacts the metabolites and bioaccessibility of calcium and carotenoids in fermented dairy products.
Food Res Int
January 2025
Department of Food Science and Nutrition, Faculty of Food Engineering, University of Campinas, Campinas, SP, Brazil. Electronic address:
This study evaluated the impact of fermentation with Lactobacillus acidophilus pre-subjected to acid, osmotic, and oxidative stress conditions on the production of metabolites and the bioaccessibility of nutrients and bioactive compounds in fermented milks and yogurts. The products were added with orange bagasse (additional calcium - Ca source) and buriti pulp (carotenoids source). Gas chromatography coupled with mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) were used to analyze the volatile and non-volatile compounds metabolites from fermentation, respectively.
View Article and Find Full Text PDFDifferences in bacterial community structure and volatile flavor substance of industrial-scale tiger skin chicken feet stored at air packaging and vacuum packaging.
Food Res Int
January 2025
Anhui Wangxiaolu Food Technology Co. Ltd, Chuzhou 239000, China. Electronic address:
The aim of this study was to investigate the differences of quality indexes, bacterial community and volatile organic compounds (VOCs) of industrial-scale tiger skin chicken feet (TSCF) under air packaging (AP) and vacuum packaging (VP). The results showed that the pH, total volatile basic nitrogen, total number of bacterial colony, and sensory scores in VP group changed less than those in AP group during the storage period. Different packaging conditions also had significant effects on bacterial community at the genus levels.
View Article and Find Full Text PDFA Health Risk Assessment of Workers Exposed to Organic Paint Solvents Used in the Korean Shipbuilding Industry.
Toxics
December 2024
Health and Safety Convergence Science Introduction, College of Health Science, Korea University, Seoul 02841, Republic of Korea.
In the shipbuilding industry, during the painting process, workers are exposed to various substances in paint, including organic solvents that can adversely affect their health. Most workplace exposures to organic solvents involve mixtures of organic compounds. Therefore, in this study, the hazard quotient (HQ) and hazard index (HI) were derived using data from the Workplace Environmental Monitoring Program in Korea for six organic solvents (xylene, n-butanol, ethylbenzene, isobutyl alcohol, toluene, and methylisobutyl ketone [MIBK]) commonly used in the steel shipbuilding industry.
View Article and Find Full Text PDFEnhanced butanol tolerance and production from puerariae slag hydrolysate by Clostridium beijerinckii through metabolic engineering and process regulation strategies.
Bioresour Technol
January 2025
College of Bioscience and Bioengineering, Institute of Applied Microbiology, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China. Electronic address:
Article Synopsis
- Butanol is identified as a valuable second-generation biomass energy source, but its production faces challenges like toxicity to fermentation strains and high feedstock costs.
- The study focuses on enhancing butanol tolerance and production in the strain Clostridium beijerinckii D9 through metabolic engineering, leading to the development of a more efficient strain, D9/pykA.
- Results showed that the new strain achieved a significant increase in butanol and total solvent production compared to the original strain, with optimal conditions boosting yield and revealing mechanisms to improve fermentation efficiency.
Optimization, characterization, and GC-MS analysis of recycled used engine oil by solvents and adsorbent extraction.
Sci Rep
January 2025
Department of Mechanical Engineering, Adama Science and Technology University, Adama, Ethiopia.
Recycled engine oil was produced from used engine oil (15W-40) using a combination of MEK and 1-butanol solvents with an activated carbon adsorbent through the adsorption process. The experimental design matrix was prepared using Box-Behnken design (BBD) package within RSM, and recycled engine oil is optimized by response surface methodology (RSM). By varying solvent-to-oil ratio, the temperature and contact duration optimal conditions were identified as a 5.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!