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The use of countercurrent chromatography in the separation of nonpolar lipid compounds. | LitMetric

The use of countercurrent chromatography in the separation of nonpolar lipid compounds.

J Chromatogr A

University of Hohenheim, Institute of Food Chemistry, Department of Food Chemistry (170b), D-70593 Stuttgart, Germany.

Published: June 2017

AI Article Synopsis

  • Isolating lipophilic compounds using countercurrent chromatography (CCC) is challenging due to the difficulty in obtaining suitable biphasic solvent systems.
  • The study isolates conjugated linolenic acids (CLnAs) from pomegranate oil and tocotrienols from palm oil using specific solvent systems, achieving high purity levels.
  • The article also discusses the advantages and disadvantages of using benzotrifluoride (BTF) in solvent systems compared to simpler alternatives for isolating other compounds like phytosterols.

Article Abstract

Isolation of lipophilic compounds by countercurrent chromatography (CCC) is a challenge because biphasic solvent systems in which these compounds distribute evenly are difficult to obtain. In this article we present novel applications of lipid compound isolation from natural sources. Conjugated linolenic acids (CLnAs, log K ∼7) were isolated from pomegranate oil using a solvent system consisting of n-heptane/methanol/water 100:91:9 (v/v/v). The CLnA fraction was free of other fatty acids but consisted of different isomers which were not resolved from each other. In the less polar range (log K ∼12), three tocotrienols (α-, γ- and δ-tocotrienol) were isolated from a vitamin E capsule produced from palm oil by using the solvent system n-hexane/acetonitrile/benzotrifluoride (BTF) at a ratio of 10:6.5:3.5 (v/v/v). Between 36 and 65mg of each of the three tocotrienols were obtained in one injection with purities >97%. Advantages and disadvantages of the "BTF system" are discussed by comparing the phase composition with the simple n-hexane/acetonitrile system and by the fractionation of phytosterols (log K ∼9.5) from rapeseed oil.

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Source
http://dx.doi.org/10.1016/j.chroma.2017.04.035DOI Listing

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