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Pregnenolone and progesterone production from natural sterols using recombinant strain of Mycolicibacterium smegmatis mc 155 expressing mammalian steroidogenesis system.
Microb Cell Fact
April 2024
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, RAS, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", Pushchino, 142290, Russia.
Article Synopsis
- Pregnenolone and progesterone are crucial steroid hormones in mammals, and new recombinant strains are needed for more efficient microbiological production from sterols.
- A new strain of Mycolicibacterium smegmatis was developed to express steroidogenic enzymes from the bovine adrenal cortex, leading to the production of progesterone and pregnenolone from cholesterol.
- The study demonstrated successful transformation of sterols under optimized conditions, yielding high-purity crystalline pregnenolone, which enhances the potential for efficient hormone production using microbial platforms.
Crystal Structure and Biochemical Analysis of a Cytochrome P450 Steroid Hydroxylase (CYP106A6) from Species.
J Microbiol Biotechnol
March 2023
Department of Life Science and Biochemical Engineering, Sunmoon University, Asan 31460, Republic of Korea.
Cytochrome P450 (CYP) is a heme-containing enzyme that catalyzes hydroxylation reactions with various substrate molecules. Steroid hydroxylases are particularly useful for effectively introducing hydroxyl groups into a wide range of steroids in the pharmaceutical industry. This study reports a newly identified CYP steroid hydroxylase (CYP106A6) from the bacterium sp.
View Article and Find Full Text PDFProgesterone in the Brain: Hormone, Neurosteroid and Neuroprotectant.
Int J Mol Sci
July 2020
U 1195 Inserm and University Paris Saclay, University Paris Sud, 94276 Le kremlin Bicêtre, France.
Progesterone has a broad spectrum of actions in the brain. Among these, the neuroprotective effects are well documented. Progesterone neural effects are mediated by multiple signaling pathways involving binding to specific receptors (intracellular progesterone receptors (PR); membrane-associated progesterone receptor membrane component 1 (PGRMC1); and membrane progesterone receptors (mPRs)) and local bioconversion to 3α,5α-tetrahydroprogesterone (3α,5α-THPROG), which modulates GABA receptors.
View Article and Find Full Text PDFHydroxylation of Steroids by a Microbial Substrate-Promiscuous P450 Cytochrome (CYP105D7): Key Arginine Residues for Rational Design.
Appl Environ Microbiol
December 2019
College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, China
Our previous study showed that CYP105D7, a substrate-promiscuous P450, catalyzes the hydroxylation of 1-deoxypentalenic acid, diclofenac, naringenin, and compactin. In this study, 14 steroid compounds were screened using recombinant cells harboring genes encoding CYP105D7 and redox partners (Pdx/Pdr, RhFRED, and FdxH/FprD), and the screening identified steroid A-ring 2β- and D-ring 16β-hydroxylation activity. Wild-type CYP105D7 was able to catalyze the hydroxylation of five steroids (testosterone, progesterone, 4-androstene-3,17-dione, adrenosterone, and cortisone) with low (<10%) conversion rates.
View Article and Find Full Text PDFBaeyer-Villiger oxidation of progesterone by Aspergillus sojae PTCC 5196.
Steroids
December 2018
Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran 1417614411, Iran.
Microbial transformations are capable of producing steroid substances difficult to synthesize by chemical methods. Strains belonging to the genus Aspergillus are effective facilitators of microbial biotransformations due to their enzymatic diversity. In this study, the biotransformation of progesterone by the fungus Aspergillus sojae (A.
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