The reversible N-methyladenosine (mA) modification of eukaryotic messenger RNAs (mRNAs) is a widespread regulatory mechanism that impacts every step in the mRNA life cycle. The effect of mA on mRNA fate depends on the binding of "mA reader" proteins - RNA binding proteins that specifically bind to RNAs containing mA. Here, we describe an RNA pull-down method that can be used to identify novel mA reader proteins starting from a known mA-modified site in cellular or viral RNA. We further describe how a combination of immunoprecipitation-based sequencing methods can be used to identify mA-modified sites bound by an mA reader protein on a transcriptome-wide level. The discovery of new mA reader proteins and their mA-modified targets would provide further insight into the mechanisms and functions of mA in the cell.
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| http://dx.doi.org/10.1016/j.ymeth.2017.04.019 | DOI Listing |
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