AI Article Synopsis
- EHV-1 is a viral infection in horses that can lead to respiratory issues, abortion, and neurological problems; a study analyzed 38 sequences from equine abortion cases in Poland from 1999 to 2016.
- 81.6% of the sequences did not fit into any known groups, while a small percentage belonged to groups III (7.9%) and IV (10.5%) based on the ORF68 gene.
- The study concluded that the ORF68 gene is not a reliable marker for tracking EHV-1 globally, but the predominant cause of abortions in Poland during the study period was linked to the ORF30 N genotype, with no rise in the related ORF30 D variant.
Article Abstract
Equid herpesvirus type 1 (EHV-1) is a common viral infection associated with varied clinical outcomes including respiratory disease, abortion and neurological disease. We have characterized EHV-1 sequences (n = 38) obtained from cases of equine abortion in Poland between 1999 and 2016, based on sequencing of PCR products from open reading frames (ORF) 30 and 68 of the EHV-1 genome. The majority (81.6%) of sequences were not classified into any of the previously described groups based on the ORF68 sequence. The remaining sequences belonged to ORF68 group III (7.9%) or IV (10.5%). A haplotype network analysis did not show any obvious structure within networks of local Polish sequences, nor within a global network of 215 EHV-1 sequences when these networks were coloured based on the geographical origin of viruses or date of detection. Our data suggest that ORF68 does not provide a reliable molecular marker for epidemiological studies of EHV-1, at least in a global sense. Its usefulness to aid local investigations of individual outbreaks remains to be established. All but two Polish EHV-1 sequences belonged to the ORF30 N genotype. The two ORF30 D viruses were obtained from abortion cases in 2009 and 2010. Hence, abortion cases that occurred in Poland between 1999 and 2016 were caused predominantly by EHV-1 with the ORF30 N genotype, with no indication of an increase in the prevalence of the ORF30 D variant.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5506511 | PMC |
| http://dx.doi.org/10.1007/s00705-017-3376-3 | DOI Listing |
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