Activity-based assessment of an engineered hyperthermophilic protein as a capture agent in paper-based diagnostic tests.

Mol Syst Des Eng

Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA.

Published: December 2016

Antibodies have traditionally served as the affinity reagents of choice in point-of-care diagnostic biosensors. However, this class of proteins is not ideally suited for this use, being poorly characterized and prone to thermal denaturation. Here, we present an activity-based assessment of an alternative engineered binding protein in a cellulose-based assay.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5403157PMC
http://dx.doi.org/10.1039/C6ME00032KDOI Listing

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