AI Article Synopsis
- The study aimed to assess how platelet-derived growth factor (PDGF-BB) influences the growth of specific blood cell precursors (CFU-GM and CFU-MK) and its ability to prevent cell death in CHRF cells.
- Researchers conducted experiments using both mouse and human bone marrow cells, observing the effects of different PDGF-BB concentrations on cell growth and survival.
- Results showed that PDGF-BB can significantly boost the proliferation of CFU-GM and CFU-MK up to a certain level (50 ng/ml being the most effective), and it also reduced apoptosis in CHRF cells.
Article Abstract
Objective: To investigate the effect of platelet-derived growth factor (PDGF-BB) on the formation of granulocyte-monocyte colony forming unit (CFU-GM) and megakaryocyte colony forming unit (CFU-MK) and its anti-apoptotic effect on CHRF cells.
Methods: The CFU-GM and CFU-MK of murine and human bone marrow cells were cultured in vitro by using plasma clot culture system. The anti-apoptotic effect of PDGF-BB on CHRF cells and its mechanism were clarified by flow cytometry.
Results: PDGF-BB 0-100 ng/ml stimulated the proliferation of murine and human CFU-GM and CFU-MK in a dose-dependent manner. The maximal stimulation effect was obtained at 50 ng/ml of PDGF-BB (P<0.01). Furthermore, PDGF-BB had an anti-apoptotic effect on CHRF cells as shown by the flow cytometry with AnnexinV/PI double staining, Caspase-3 expression and JC-1 detection (P<0.05).
Conclusion: PDGF-BB significantly stimulates the proliferation of CFU-GM and CFU-MK in vitro, and has an anti-apoptotic effect on CHRF cells.
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| http://dx.doi.org/10.7534/j.issn.1009-2137.2017.02.044 | DOI Listing |
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