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Real time plasmonic qPCR: how fast is ultra-fast? 30 cycles in 54 seconds. | LitMetric

Real time plasmonic qPCR: how fast is ultra-fast? 30 cycles in 54 seconds.

Analyst

Lady Davis Institute for Medical Research - Jewish General Hospital, Montreal, QC, Canada. and Department of Medicine, McGill University, Montreal, QC, Canada.

Published: May 2017

AI Article Synopsis

  • PCR is a vital tool in biological research and is increasingly used in clinical, veterinary, and agricultural settings.
  • Plasmonic PCR uses metallic nanoparticles for efficient heat transfer, allowing for rapid PCR reactions and scalability in various applications, from portable diagnostic devices to larger laboratory setups.
  • Recent advancements enable a 30-cycle PCR in just 54 seconds, enhancing real-time monitoring capabilities and significantly improving rapid diagnostics in point-of-care settings.

Article Abstract

Polymerase Chain Reaction (PCR) is a critical tool for biological research investigators but recently it also has been making a significant impact in clinical, veterinary and agricultural applications. Plasmonic PCR, which employs the very efficient heat transfer of optically irradiated metallic nanoparticles, is a simple and powerful methodology to drive PCR reactions. The scalability of next generation plasmonic PCR technology will introduce various forms of PCR applications ranging from small footprint portable point of care diagnostic devices to large footprint central laboratory multiplexing devices. In a significant advance, we have introduced a real time plasmonic PCR and explored the ability of ultra-fast cycling compatible with both label-free and fluorescence-based monitoring of amplicon production. Furthermore, plasmonic PCR has been substantially optimized to now deliver a 30 cycle PCR in 54 seconds, with a detectable product. The advances described here will have an immediate impact on the further development of the use of plasmonic PCR playing a critical role in rapid point of care diagnostics.

Download full-text PDF

Source
http://dx.doi.org/10.1039/c7an00304hDOI Listing

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