A Novel PCR Assay for Detecting and .

Objectives: Brucellosis is a major zoonotic disease that poses a significant public health threat worldwide. The classical bacteriological detection process used to identify spp. is difficult and time-consuming. This study aimed to develop a novel molecular assay for detecting brucellosis.

Methods: All complete sequences of chromosome 1 with 2.1-Mbp lengths were compared among all available sequences. A unique repeat sequence (URS) locus on chromosome 1 could differentiate from . A primer set was designed to flank the unique locus. A total of 136 lymph nodes and blood samples were evaluated and classified by the URS-polymerase chain reaction (PCR) method in 2013-2014.

Results: Biochemical tests and bacteriophage typing as the golden standard indicated that all spp. isolates were biovar 1 and biovar 3. The PCR results were the same as the bacteriological method for detecting spp. The sensitivity and specificity of the URS-PCR method make it suitable for detecting and .

Conclusion: Quick detection of and can provide the most effective strategies for control of these bacteria. The advantage of this method over other presented methods is that both and are detectable in a single test tube. Furthermore, this method covered 100% of all and biotypes. The development of this URS-PCR method is the first step toward the development of a novel kit for the molecular identification of and .