Premise Of The Study: Transcriptome-derived simple sequence repeat (SSR) markers were developed in (Asparagaceae). Due to its application in traditional medicine, its wild populations are threatened by over-collection even in protected areas, requiring immediate conservation efforts.
Methods And Results: Based on transcriptome data of , 96 primer pairs with two to seven alleles per locus were selected for initial validation; of those, 27 primer pairs amplified across all samples, resulting in 15 polymorphic and 12 monomorphic microsatellite markers. The usefulness of these markers was assessed in 60 individuals representing three populations of . Observed and expected heterozygosity values ranged from 0.050 to 0.950 and 0.049 to 0.626, respectively. Cross-species amplification of the 27 markers was tested in the related species . and .
Conclusions: These polymorphic, transcriptome-derived SSR markers can be used as molecular markers to study population genetics and ecological conservation in and related taxa.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5400436 | PMC |
| http://dx.doi.org/10.3732/apps.1700021 | DOI Listing |
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