A sensitive and convenient flow-injection chemiluminescence (FI-CL) turn-on assay for alkaline phosphatase (ALP) activity without any label and synthesis is developed. Cu can catalyze the luminol-H O CL reaction. Pyrophosphate (PPi) can chelate Cu and therefore the Cu -mediated luminol-H O CL reaction is inhibited. The addition of ALP can catalyze the hydrolysis of PPi into phosphate ions, Cu is released and the chemiluminescence recovers. A detection limit of 1 mU/mL ALP is obtained.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/bio.3302 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Sub-Acute Potential Risk of Oxamyl in Male Albino Rats.
Environ Toxicol
December 2024
Pesticide Chemistry & Technology Department, Faculty of Agriculture, Alexandria University, Alexandria, Egypt.
The current study aimed to investigate the sub-acute effects of oxamyl on male Albino rats following oral administration of either 0.031 or 0.31 mg/kg/day for 14 consecutive days.
View Article and Find Full Text PDFPerioperative risk factors for osteoporosis after radical gastrectomy for gastric cancer.
BMC Surg
December 2024
Department of Surgery, Seoul Metropolitan Government-Seoul National University Boramae Medical Center, Boramae-ro 5-20, Dongjak-gu, Seoul, 07061, Republic of Korea.
Background: Osteoporosis, a frequent complication of gastrectomy, increases with age, and the average age of gastric cancer patients continues to rise. This study aims to analyze perioperative factors of osteoporosis after radical gastrectomy.
Materials And Methods: This retrospective cohort study included patients who underwent dual-energy-X-ray absorptiometry after gastrectomy due to gastric cancer between 2016 and 2019 at Seoul Boramae Medical Center.
Safety assessment on CBD-rich hemp extract in sub-chronic cross-sex study with rats.
Toxicol Appl Pharmacol
December 2024
Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic. Electronic address:
Cannabidiol (CBD) is a phytocannabinoid from Cannabis sativa L., in which there is currently growing interest for medicinal use. Here, we focused on the safety and pharmacokinetics of a CBD-rich (77 %, w/w) full-spectrum hemp extract in male and female rats.
View Article and Find Full Text PDFEvaluation of Serum Lipids, Biochemical Parameters, Selected Antioxidant Elements and Oxidative Stress Profiles in Late Pregnant Jennies with Hyperlipemia.
Vet Sci
December 2024
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
Donkeys are particularly at risk of hyperlipemia. Hyperlipemia is a metabolic disease caused by the mobilization of fatty acids from adipose tissue, which often impacts pregnant and lactating jennies (female donkeys) during periods of negative energy balance. This study aimed to evaluate the levels of lipids, biochemical parameters, selected antioxidant elements and oxidative stress parameters in late pregnant jennies affected by hyperlipemia.
View Article and Find Full Text PDFA Multi-Enzyme Complex That Mitigates Hepatotoxicity, Improves Egg Production and Quality, and Enhances Gut and Liver Health in Laying Hens Exposed to Trace Aflatoxin B.
Toxins (Basel)
November 2024
Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
Aflatoxin B is a prevalent secondary hazardous metabolite generated by fungus present in feed ingredients and the surrounding environment: enzymes are currently being recognized as an efficient and promising approach to reducing the associated risks. The objective of this study was to assess the effects of varying doses of enzyme complexes on several parameters in laying hens that were exposed to aflatoxin. During an 8-week experiment, a total of 288 Yukou Jingfen No.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!