A new method of solid-phase analysis of viral antigens in which St. aureus, strain Cowan 1, was used as a label is described. The result of analysis is read visually by bacterial agglutination. The degree of agglutination of the staphylococcal diagnosticum corresponds to the amount of the antigen bound with antibodies immobilized on the solid phase. Staining of Staphylococcus with methylene blue simplifies reading of the results. The method has been shown to be sufficiently simple, specific, sensitive, and may be used for diagnosis of different infections diseases.
Download full-text PDF |
Source |
---|
J Vet Res
December 2024
Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Puławy, Poland.
Introduction: Synthetic anabolic hormones, which may pose a potential risk to human health, should not be used in fattening food-producing animals. Because of the hormonal effects they cause, growth-promoting compounds are banned by legislation in the EU for use in animal husbandry. Consequently, all EU member states are required to conduct monitoring tests on the content and residues of these compounds in prescribed biological matrices to ensure the safety of food consumers.
View Article and Find Full Text PDFMycopathologia
January 2025
Laboratory of Fungal Genetics and Metabolism, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic.
The clinical diagnosis of dermatophytosis and identification of dermatophytes face challenges due to reliance on culture-based methods. Rapid, cost-effective detection techniques for volatile organic compounds (VOCs) have been developed for other microorganisms, but their application to dermatophytes is limited. This study explores using VOCs as diagnostic markers for dermatophytes.
View Article and Find Full Text PDFLab Chip
January 2025
Department of Food Science and Agricultural Chemistry, McGill University Macdonald Campus, Sainte-Anne-de-Bellevue, Quebec, H9X 3V9, Canada.
Mycotoxins are detectable in 60-80% of food crops, posing significant threats to human health and food security, and causing substantial economic losses. Most mitigation approaches focus on detecting mycotoxins with standard methods based on liquid chromatography coupled with mass spectrometry (LC-MS). Typical MS methods require extensive sample preparation and clean-up due to the matrix effect, followed by time-consuming LC separation, complicating the analysis process and limiting analytical throughput.
View Article and Find Full Text PDFToxics
December 2024
School of Resource and Environmental Engineering, Shandong University of Technology, Zibo 255000, China.
The solid phase composition in oily sludge (OS) is a key factor affecting the oil-solid separation of OS. In this paper, the effects and mechanisms of solid-phase particle factors on the oil content of residue phase were investigated in order to improve the oil-solid separation efficiency. Flotation experiments were carried out on single-size sand and mixed-size sand OS consisting of three particle sizes at room temperature without adding flotation reagents.
View Article and Find Full Text PDFToxics
November 2024
Department of Food Analysis and Nutrition, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Technicka 3, 166 28 Prague, Czech Republic.
The level of the human body's burden of benzophenone and camphor ultraviolet (UV) filters can be estimated from their urinary levels. The present study describes the implementations and validation of the sensitive analytical method for the analysis of seven benzophenone and two camphor UV filters in urine. Sample preparation includes overnight enzymatic hydrolysis and ethyl acetate extraction followed by purification by dispersive solid-phase extraction using a sorbent Z-Sep.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!