A PHP Error was encountered

Severity: Warning

Message: file_get_contents(https://...@gmail.com&api_key=61f08fa0b96a73de8c900d749fcb997acc09): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests

Filename: helpers/my_audit_helper.php

Line Number: 143

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 143
Function: file_get_contents

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 209
Function: simplexml_load_file_from_url

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3098
Function: getPubMedXML

File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: Attempt to read property "Count" on bool

Filename: helpers/my_audit_helper.php

Line Number: 3100

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3100
Function: _error_handler

File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

Immune responses to highly conserved influenza A virus matrix 1 peptides. | LitMetric

Immune responses to highly conserved influenza A virus matrix 1 peptides.

Microbiol Immunol

Department of Biotechnology, Thapar University, Patiala147004, India.

Published: June 2017

AI Article Synopsis

  • Influenza vaccine development is complicated due to constantly changing viruses, which necessitates ongoing global monitoring and vaccine reformulation.
  • Researchers examined three highly conserved matrix 1 peptides (P 1, P 2, P 3) for their ability to trigger immune responses in blood samples from healthy volunteers.
  • The findings showed that all tested peptides elicited immune responses, particularly P 3, suggesting these conserved peptides could be valuable for creating cross-protective influenza vaccines.

Article Abstract

Influenza vaccine development is considered to be complicated and challenging. Constantly evolving influenza viruses require continuous global monitoring and reformulation of the vaccine strains. Peptides that are conserved among different strains and subtypes of influenza A virus are strongly considered to be attractive targets for development of cross protective influenza vaccines that stimulate cellular responses. In this study, three highly conserved (>90%) matrix 1 peptides that contain multiple T cell epitopes, ILGFVFTLTVPSERGLQRRRF (P 1), LIRHENRMVLASTTAKA (P 2) and LQAYQKRMGVQMQR (P 3), were assessed for their immunogenic potential in vitro by subjecting peripheral blood mononuclear cells from healthy volunteers to repetitive stimulation with these chemically synthesised peptides and measuring their IFN-γ concentrations, proliferation by ELISA, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, respectively. Seven samples were screened for immunogenicity of P 1 and P 2, and six for that of P 3. All six samples had positive responses (IFN-γ secretion) to P 3 stimulation, as did five and three for P 2 and P 1 respectively. In contrast, seven (P 1 and P 2) and four (P 3) samples showed proliferative response as compared with unstimulated cells. The encouraging immunogenic response generated by these highly conserved matrix 1 peptides indicates they are prospective candidates for development of broadly reactive influenza vaccines.

Download full-text PDF

Source
http://dx.doi.org/10.1111/1348-0421.12485DOI Listing

Publication Analysis

Top Keywords

highly conserved
12
matrix peptides
12
influenza virus
8
influenza vaccines
8
influenza
6
peptides
5
immune responses
4
responses highly
4
conserved
4
conserved influenza
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!

A PHP Error was encountered

Severity: Notice

Message: fwrite(): Write of 34 bytes failed with errno=28 No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 272

Backtrace:

A PHP Error was encountered

Severity: Warning

Message: session_write_close(): Failed to write session data using user defined save handler. (session.save_path: /var/lib/php/sessions)

Filename: Unknown

Line Number: 0

Backtrace: