The aim of this study is to investigate in vivo anti-rheumatic activity of methotrexate-entrapped ultradeformable liposomal gel (MTX-UDLs-gel) in adjuvant-induced arthritis rat model. Methotrexate-entrapped ultradeformable liposomes (MTX-UDLs) with the optimal phosphatidylcholine to Tween 80 ratio (7:3, w/w) were incorporated into 1% Carbopol gel. MTX-UDLs-gel was characterized in terms of appearance, clarity, homogeneity, pH and drug content. The permeation of MTX-UDLs-gel across rat skin was investigated using Franz diffusion cell. In vivo anti-rheumatic activity of MTX-UDLs-gel was assessed in terms of edema volume, paw edema and leukocyte infiltration scores, histopathological analysis and inflammatory cytokines level in complete Freund's adjuvant (CFA)-induced arthritis rat model. MTX-UDLs-gel showed good homogeneity and clarity, neutral pH and about 99.5% drug content. The cumulative amount of MTX permeated for 24h from MTX-UDLs-gel (164.6μg) was 1.5 and 2.15 times higher than that of MTX-CLs-gel (113.3μg) and MTX-plain-gel (76.6μg), respectively. MTX-UDLs-gel significantly alleviated the severity of inflammation by reducing edema volume, histological scores and accumulation of neutrophils and improving tissue architecture in CFA-induced arthritis rat model. MTX-UDLs-gel effectively suppressed the expression of pro-inflammatory cytokines, TNF-α and IL-1β, in paw tissues. In conclusion, the developed MTX-UDLs-gel has a great potential for effective delivery of MTX into the inflamed joints in rheumatoid arthritis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijpharm.2017.04.032 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Ameliorative effect of venom against arthritic influence of Freund's complete adjuvant FCA in paw and ankle of male rats.
Open Vet J
November 2024
Zoology Department, Faculty of Science, Zagazig University, Zagazig, Egypt.
Background: Pain and inflammation are closely associated with rheumatoid arthritis (RA), which affects the bones and joints.
Aim: While there are a number of therapeutic options for arthritis, their side effects restrict their use and encourage the search for alternative, natural remedies.
Methods: In male rats, we examined the anti-inflammatory and anti-arthritic properties of venom (NHV).
The effect of Miya on skeletal muscle changes by regulating gut microbiota in rats with osteoarthritis through AMPK pathway.
BMC Musculoskelet Disord
December 2024
Department of Orthopedics, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Rd, Shanghai, 200072, People's Republic of China.
Background: The study aimed to explore whether Miya (MY), a kind of Clostridium butyricum, regulated osteoarthritis (OA) progression through adenosine 5'-monophosphate-activated protein kinase (AMPK) pathway.
Methods: The OA rats were orally given MY daily for 4 weeks and were intramuscularly injected with AMPK inhibitor once a week for 4 weeks. Hematoxylin eosin (HE) staining was used to observe the histological morphology of the knee joint.
Investigating the mechanism of Tongfeng Qingxiao Formula for improving gouty arthritis based on the neutrophil extracellular trapping network.
J Ethnopharmacol
December 2024
Graduate School, Jiangxi University of Chinese Medicine, Nanchang 330004, China; Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, China. Electronic address:
Ethnopharmacological Relevance: Traditional Chinese Medicine (TCM) has a history of thousands of years in China. The Tongfeng Qingxiao Formula (TFQXF), after decoction and oral administration, has shown significant therapeutic effects on Gouty arthritis (GA). TCM is often considered an experiential medicine, lacking modern scientific research, and the efficacy of TFQXF faces the same issue.
View Article and Find Full Text PDFTocilizumab does not ameliorate inflammation-induced left ventricular dysfunction in a collagen-induced arthritis rat model.
Cardiovasc Pathol
December 2024
Wits Integrated Molecular Physiology Research Initiative, Wits Health Consortium (PTY) Ltd, School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, 2193 Johannesburg, South Africa.
Background: Interleukin-6 (IL-6) is an attractive therapeutic target due to its diverse roles in the pathogenesis of conditions characterized by systemic inflammation. IL-6 has also been implicated in the pathophysiology of heart failure. This study aimed to investigate the impact of IL-6 receptor blockade with tocilizumab on the molecular pathways underlying systemic inflammation-induced left ventricular (LV) dysfunction in a collagen-induced arthritis (CIA) rat model.
View Article and Find Full Text PDFIntegrating lipid metabolomics, serum medicinal chemistry, network pharmacology and experimental validation to explore the mechanism of Sanmiao wan in the treatment of rheumatoid arthritis.
J Ethnopharmacol
December 2024
Mudanjiang Normal University, Mudanjiang,157011, China.
Ethnopharmacological Relevance: Rheumatoid arthritis (RA) is a common autoimmune disease with a high clinical morbidity and leads to persistent chronic inflammation. Sanmiao wan is a classic formula for the treatment of RA, and the results of clinical and experimental studies have shown its therapeutic effect on RA. However, its mechanism of action remains unclear.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!