Ultrason Sonochem
School of Food & Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China. Electronic address:
Published: July 2017
Here we report the enhancement of both cellular permeability and cell growth rate of Candida tropicalis after treatment with the ultrasonic irradiation of low intensity using a mode of sweeping frequency (UILS) generated by a self-developed ultrasonic device in our lab. After the ultrasonic treatment, remarkable biomass enhancement of the yeast was observed; the hyphae became significantly longer; the seeped cellular protein and nucleic acid from the yeast increased and the cellular Ca content became lower. Illumina transcriptome sequencing showed that the ultrasonic treatment affected the expression of genes involved in diverse cellular components, biological processes and molecular functions. RT-PCR and Western blotting further confirmed the up-/down-regulation of genes in the ultrasound-treated yeasts. The optimal conditions of the ultrasonic treatment for the maximum biomass addition were determined as follows: the yeast was treated for 1h at the mid logarithmic phase, the frequency was 28±2kHz and the power density was 120W/L. Under these conditions, the Candida tropicalis biomass increased by 142.5% compared with the untreated yeast.
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http://dx.doi.org/10.1016/j.ultsonch.2017.02.010 | DOI Listing |
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