AI Article Synopsis
- Researchers are building a detailed genetic database of medieval Hungarian conquerors by resequencing the entire mitochondrial DNA (mtDNA) of 24 ancient samples using advanced sequencing technology.
- Traditional PCR methods used for genetic sequencing often lead to incorrect identification of haplotypes and haplogroups due to their susceptibility to ambiguous sequence reads.
- The SNaPshot method, combined with established criteria for ancient DNA authenticity, offers a cost-effective and efficient way to analyze coding region SNPs for accurate haplogroup classification.
Article Abstract
As part of the effort to create a high resolution representative sequence database of the medieval Hungarian conquerors we have resequenced the entire mtDNA genome of 24 published ancient samples with Next Generation Sequencing, whose haplotypes had been previously determined with traditional PCR based methods. We show that PCR based methods are prone to erroneous haplotype or haplogroup determination due to ambiguous sequence reads, and many of the resequenced samples had been classified inaccurately. The SNaPshot method applied with published ancient DNA authenticity criteria is the most straightforward and cheapest PCR based approach for testing a large number of coding region SNP-s, which greatly facilitates correct haplogroup determination.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5396865 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0174886 | PLOS |
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