A 250-kDa protein was isolated from fluid in the middle spermatic duct (MSD) of the blue crab (Portunus pelagicus). N-terminal and partial amino acid sequences revealed that this MSD-specific protein is highly similar to the plasma-enriched protein Alpha-2 macroglobulin (α2M). The P. pelagicus ortholog (Ppα2M) is a large glycoprotein possessing mannose and N-acetylglucosamine residues. Ppa2m mRNA was detected in the spermatic duct, androgenic gland, and hematopoietic tissue, whereas the protein was primarily observed in the apical cytoplasm of MSD epithelium and in the matrix of the acrosome of MSD sperm; distally within spermatic duct, Ppα2M was lost from the sperm membrane but remained in the sperm acrosome. These results suggest that Ppα2M is expressed and glycosylated in the epithelium of spermatic ducts, secreted into MSD fluid, taken up by sperm in the MSD, and removed from the surface of sperm during its transit towards the female spermatheca. Given that Ppα2M also exhibits protease inhibitor activity, we hypothesize that acrosome localized Ppα2M may suppress premature acrosome reaction during post-testicular sperm maturation in this crab.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/mrd.22817 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Isolated spermatic cord metastasis of prostate cancer after radiotherapy detected with F-PSMA PET/CT: a case report and literature review.
Int Cancer Conf J
October 2024
Division of Nuclear Medicine, Department of Radiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Article Synopsis
- * Imaging with F-PSMA PET/CT revealed a cancerous lesion in the left spermatic cord, which was confirmed as prostatic adenocarcinoma after surgery.
- * The exact cause of the metastasis is unclear, but potential pathways include intraluminal, hematogenous, and lymphatic routes; the case emphasizes the effectiveness of F-PSMA PET/CT in identifying cancer recurrence in unusual locations, even with low PSA levels.
EphB2, EphB4, and ephrin-B1 expression and localization in postnatal developing epididymis in mice.
Dev Dyn
October 2024
Laboratory of Veterinary Anatomy, Graduate School of Veterinary Science, Osaka Metropolitan University, Izumisano, Osaka, Japan.
Background: Eph receptors and ephrin ligands, the transmembrane proteins, function as a mechanism of communication between cells. Therefore, we intended to explore the expression array of EphB2 and EphB4 receptors and ephrin-B1 ligand in postnatal developing mouse epididymis during 1 day to 8 weeks using RT-PCR amplification and immunofluorescence staining.
Results: RT-PCR analysis indicated that the expression levels of EphB2, EphB4, and ephrin-B1 in the epididymis declined with the advancement of age during the initial phases of postnatal development and stayed relatively near to adult levels until 4 weeks.
Morphology of the Female Receptaculum Seminis of Euschistus heros (FABRICIUS, 1798) (INSECTA: HEMIPTERA: PENTATOMIDAE).
An Acad Bras Cienc
October 2024
Universidade Federal de Viçosa, Departamento de Biologia Geral, Avenida Peter Henry Rolfs, s/n, 36570-000 Viçosa, MG, Brazil.
Euschistus heros (Fabricius, 1798) (Heteroptera: Pentatomidae) is a soybean pest in Brazil. It has a reproductive success that may be associated with the female receptaculum seminis or spermatheca, a reproductive organ for spermatozoa storage until the oocyte fertilization. This study describes the anatomy and histology of the female receptaculum seminis in E.
View Article and Find Full Text PDFThe spermatheca ultrastructure of the ground beetle Clinidium canaliculatum (Costa) (Carabidae, Rhysodinae).
Micron
December 2024
Department of Life Sciences, University of Siena, Siena, Italy. Electronic address:
The ground beetle Clinidium canaliculatum is a member of Rhysodinae, a taxon with still discussed systematic position. The spermatheca of this species is a small cylindrical structure connected to the common oviduct by a thin duct. The ultrastructure of the organ has revealed that the apical receptacle is provided with an epithelium lined by a thick cuticle from the deeper region of which several finger-like cuticular structures extend into the cytoplasm.
View Article and Find Full Text PDFTesticular tumor in a case of, undescended testes, persistent mullerian duct syndrome and transverse testicular ectopia: Report of a case and review of the literature.
Urol Case Rep
September 2024
Urology and Kidney Transplantation Department, Ibn Senna Hospital, Sudan Medical Specialization Board of Urology, University of Khartoum, Faculty of Medicine, Anatomy Department, P.O BOX 102, Qasr Street, Khartoum, 11111, Sudan.
A 20-year-old with normal male body features and secondary sexual characteristics presented with a right testicular swelling. Imaging revealed a right testicular mass, leading to a diagnosis of classical seminoma. During inguinal orchiectomy, a solid testicular mass was found on the right side along with two spermatic cords, one attached to the mass and the other to a structure resembling a testes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!