AI Article Synopsis
- Immunocytochemistry helps identify specific antigens in tissues, but accessing certain areas in plant cell walls can be challenging due to their complex structures.
- Carbohydrate-binding modules (CBMs) are smaller probes that can effectively navigate these complexities where larger antibodies might struggle.
- This chapter focuses on using CBMs to investigate the arrangement of polysaccharides in plant cell walls and to measure how enzymes break them down.
Article Abstract
Immunocytochemistry is a widely used technique to localize antigen within intact tissues. Plant cell walls are complex matrixes of highly decorated polysaccharides and the large number of CBM families displaying specific substrate recognition reflects this complexity. The accessibility of large proteins, such as antibodies, to their cell wall epitopes may be sometimes difficult due to steric hindrance problems. Due to their smaller size, CBMs are interesting alternative probes. The aim of this chapter is to describe the use of CBM as probes to explore complex polysaccharide topochemistry in muro and to quantify enzymatic deconstruction.
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| http://dx.doi.org/10.1007/978-1-4939-6899-2_14 | DOI Listing |
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