The quantitation of liberated reducing sugars by the copper-bicinchoninic acid (BCA) assay provides a highly sensitive method for the measurement of glycoside hydrolase (GH) activity, particularly on soluble polysaccharide substrates. Here, we describe a straightforward method adapted to low-volume polymerase chain reaction (PCR) tubes which enables the rapid, parallel determination of GH kinetics in applications ranging from initial activity screening and assay optimization, to precise Michaelis-Menten analysis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-4939-6899-2_1 | DOI Listing |
Publication Analysis
Top Keywords
copper-bicinchoninic acid
8
acid bca
8
bca assay
8
low-volume parallel
4
parallel copper-bicinchoninic
4
assay glycoside
4
glycoside hydrolases
4
hydrolases quantitation
4
quantitation liberated
4
liberated reducing
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!