FliS is a cytoplasmic flagellar chaperone for the flagellin, which polymerizes into filaments outside of the flagellated bacteria. Cytoplasmic interaction between FliS and flagellin is critical to retain the flagellin protein in a monomeric form, which is transported from the cytoplasm through the flagellar export apparatus to the extracellular space for filament assembly. Defects in the FliS protein directly diminish bacterial motility, pathogenicity, and viability. Although the overall structure of FliS is known, structural and mutational studies on FliS from other bacterial species are still required to reveal any unresolved biophysical features of FliS itself or functionally critical residues for flagellin recognition. Here, we present the crystal structure of FliS from Bacillus cereus (BcFliS) at 2.0 Å resolution. FliS possesses a highly dynamic N-terminal region, which is appended to the common four-helix bundle structure. An invariant proline residue (Pro17 in B. cereus FliS) was identified in all known FliS sequences between the N-terminal region and the four-helix bundle. The N-terminal proline residue functions as a helix breaker critical for FliS dimerization and flagellin recognition.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrc.2017.04.070DOI Listing

Publication Analysis

Top Keywords

flis
13
flagellin recognition
12
crystal structure
8
flagellar chaperone
8
flis bacillus
8
bacillus cereus
8
invariant proline
8
critical flis
8
flis dimerization
8
dimerization flagellin
8

Similar Publications

Antibacterial Effect of Fermented Pomegranate Peel Polyphenols on and Its Mechanism.

Biology (Basel)

November 2024

Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.

frequently breaks out in aquatic animal breeding operations involving shrimp, and it can endanger human health through food and wound infections. The antibacterial effect and mechanism of fermented pomegranate peel polyphenols (FPPPs) on were investigated. The results indicated that FPPPs had a strong inhibitory effect on the growth of , and their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were 2 and 4 mg/mL.

View Article and Find Full Text PDF

The study aimed to analyse the association between the digit ratio in adult women and the degree of air pollution (suspended particulate matter, sulphur dioxide, nitric dioxide, benzene levels) in the place of residence of their mothers during pregnancy. The data was collected from female students in several Polish cities. Measurements were taken, and questionnaire data were collected.

View Article and Find Full Text PDF

The aim of the study was to determine the effects of replacing soybean meal with insect meal on the body weight and the chemical composition of selected muscle groups of common pheasant females and males, including the mineral composition and the amino acid profile of the thigh and breast muscles. The study was conducted on three feeding groups, namely one control and two experimental groups. In the control group, plant feed components were used, which are commonly used to feed pheasants in confined breeding facilities.

View Article and Find Full Text PDF

Although there have been some clinical observations made, the mechanistic effects on bone metabolism of whole-body cryostimulation and high-intensity interval training (HIIT), either alone or in combination, are still debated. Here, we have investigated their effects on circulating osteo-immune and bone metabolic markers (osteopontin, osteocalcin, sclerostin, dikkopf-related protein 1, and fibroblast-growth factor 23) and their potential effects on osteoblast differentiation and function, , by treating SaOS-2 osteoblast-like cells with the sera obtained from the subjects who had undergone the different interventions or untreated control subjects. Sixty-seven inactive, overweight-to-obese participants (body mass index = 31.

View Article and Find Full Text PDF
Article Synopsis
  • Bacteria use a structure called a flagellar filament, made of many subunits, to swim, but it's unclear how they control its length.
  • Deleting certain genes in polar flagellated bacteria resulted in longer flagellar filaments, suggesting that the FlaG protein helps regulate filament length by interfering with another protein, FliS, in the secretion system.
  • Both flagellar filaments and injectisome systems appear to have evolved similar protein mechanisms to control the secretion of crucial components, highlighting a convergence in bacterial adaptation strategies.
View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!