Two single nucleotide polymorphisms sites in - gene and their association with somatic cell score in Chinese Holstein cows.

J Biol Res (Thessalon)

Key Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193 China.

Published: December 2017

Background: Alpha 1-antitrypsin (α1-AT) may affect the susceptibility of mastitis in dairy cattle for its possible role in the protection of lactoferrin from proteolytic degradation in the mammary. Milk somatic cell score (SCS) is a logarithmic transformation of the milk somatic cell count widely used as an index to evaluate mastitis. To study the relationships of - gene and SCS in Chinese Holstein cows, methods of PCR-SSCP, DNA sequencing, PCR-RFLP, and CRS-PCR technologies were used to detect single nucleotide polymorphisms sites in - gene.

Results: Two polymorphic sites at G5503A and G5746C of - gene were found. AA (0.3633), AB (0.4644) and BB (0.1723) genotypes were detected at G5503A site, CC (0.3483), CD (0.4906) and DD (0.1611) genotypes were found at G5746C in Chinese Holstein cows. Least squares mean of SCS for individuals with BB genotype was significantly lower than that with AA and AB genotype ( < 0.01), and that with AB genotype was significantly lower than that with AA ( < 0.05). There was no significant difference among individuals with CC, CD and DD genotypes ( > 0.05). Least squares mean of SCS for individuals with BBDD genotype combination were significantly lower than those with AACC and AACD ( < 0.05).

Conclusions: Statistical analysis indicated that B allele and BBDD genotype combination of α1-AT can improve mastitis resistance in dairy cattle.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390408PMC
http://dx.doi.org/10.1186/s40709-017-0065-zDOI Listing

Publication Analysis

Top Keywords

somatic cell
12
chinese holstein
12
holstein cows
12
single nucleotide
8
nucleotide polymorphisms
8
polymorphisms sites
8
cell score
8
milk somatic
8
sites gene
4
gene association
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!