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In-house made nucleofection buffer for efficient and cost effective transfection of RAW 264.7 macrophages. | LitMetric

In-house made nucleofection buffer for efficient and cost effective transfection of RAW 264.7 macrophages.

Biochem Biophys Res Commun

Department of Biology, Dr. Reddy's Institute of Life Sciences, University of Hyderabad Campus, Hyderabad, Telangana, India. Electronic address:

Published: May 2017

AI Article Synopsis

Article Abstract

Electroporation is the most widely employed method of gene transfer into macrophages which are hard to transfect. RAW 264.7 is a widely used cell line for studying macrophage responses. Electroporation of RAW 264.7 cells with commercial reagents although very efficient is expensive necessitating the development of cost effective alternatives. In this study, we have formulated an economical electroporation buffer for electroporation of RAW 264.7 cells compatible with commercial nucleofector apparatus. We observed that supplementation of membrane fusogenic agents such as Ficoll, PEG and membrane resealing agent, poloxamer P188, enhanced the transfection efficiency of macrophages to a level comparable to the commercially available solutions thereby providing us a cost effective solution for genetic manipulation of macrophages especially in large numbers.

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Source
http://dx.doi.org/10.1016/j.bbrc.2017.04.043DOI Listing

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