AI Article Synopsis
- The orthogonal pressurized planar electrochromatography (OPPEC) is a 2-D separation method combining electrophoresis and chromatography for effective substance separation.
- This technique allows for continuous input of sample solutions, efficiently separating and collecting different components based on their electrophoretic mobility.
- The paper introduces a new approach using OPPEC for substances with similar mobility, highlighting its benefits over traditional column chromatography in separating complex mixtures.
Article Abstract
The orthogonal pressurized planar electrochromatography (OPPEC) is an example of 2-D separation technique, in which two simultaneous and orthogonal processes of electrophoresis and chromatography are involved in the separation mechanism. In the case of preparative separation of substances characterized by different electrophoretic mobility, such separation system can be constantly fed with the sample solution and the separated components can be constantly collected at its outlet. In the paper, as opposed to the previous studies, we discuss the capabilities of OPPEC technique for preparative separation of substances characterized by the same electrophoretic mobility. According to the proposed solution, the separation system can be periodically fed with the sample solution and separated components can be collected alternately at its outlet. The advantages of this new approach over the column chromatography with regard to the separation of complex mixtures have been signaled.
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| http://dx.doi.org/10.1016/j.chroma.2017.03.088 | DOI Listing |
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