AI Article Synopsis
- The study introduces a novel fluorescence turn-on method for detecting alkaline phosphatase (ALP) activity using quantum dots (QDs).
- The method relies on the controlled aggregation of QDs, where adding cationic polymer (poly-1) quences their fluorescence, but ALP can reverse this by removing phosphate groups from short DNA strands.
- The detection system is sensitive and selective, allowing for the measurement of ALP at concentrations as low as 0.1 mU/mL.
Article Abstract
Based on the controlled aggregation of quantum dots (QDs), a valid, reliable, and label-free fluorescence turn-on strategy is established for the detection of alkaline phosphatase activity. The aqueous solution of anionic QDs exhibits intense fluorescence. However, the addition of cationic polymer (poly-1) significantly quenched the fluorescence of the QDs via their induced aggregation. While short 3'-phosphorylated DNA (DNA-P) could not be extended by terminal deoxynucleotidyl transferase (TdT) and therefore, fluorescence of the QDs was recovered negligibly. The effective elimination of phosphate group of DNA-P in the presence of alkaline phosphatase (ALP) produced 3'-OH termini and the resulting DNA could be sufficiently extended by TdT. The presence of greater binding strength between the elongated DNA and poly-1 is very crucial to compete with the poly-1/QDs aggregates and release the QDs. Turned-on fluorescence emission is observed due to the efficient release of the QDs. A novel strategy for alkaline phosphatase detection is therefore established. Our method is quite sensitive and selective, as low as 0.1 mU/mL ALP can be easily detected.
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| http://dx.doi.org/10.1016/j.talanta.2017.03.063 | DOI Listing |
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