AI Article Synopsis
- The study involves encapsulating Trimethylated-β-cyclodextrin (TM-β-CD) into polymer monolithic capillary columns for improved nano-liquid chromatography aimed at chiral separations.
- The monolithic phases were created through a one-pot method using specific monomers and solvents, resulting in chiral stationary phases characterized by various analytical techniques.
- Results showed that higher concentrations of TM-β-CD led to columns with larger surface area and better enantioseparation efficiency for separating racemic pharmaceuticals, highlighting the effectiveness of this encapsulation strategy.
Article Abstract
Trimethylated-β-cyclodextrin (TM-β-CD) was encapsulated within several polymer monolithic capillary columns for reversed-phase chiral nano-liquid chromatography (nano-LC). The monolithic phases were prepared using the one-pot in situ copolymerization of ethylene glycol dimethacrylate (EDMA), glycidyl methacrylate (GMA) monomers and 1-propanol, 1,4-butanediol as progenic solvents in presence of TM-β-CD solution within fused silica capillaries (150µm I.D.). The obtained chiral monolithic stationery phases were characterized by scanning electron microscopy (SEM), N adsorption/desorption isotherms, wide angle x-ray diffraction (WAXRD), thermogravimetric analysis (TGA) and differential thermal analysis (DTA). The materials characterization demonstrated that monolithic phases with higher concentration of TM-β-CD have relatively larger surface area, smaller pore size and larger total pore volume compared to those with lower concentration TM-β-CD. The prepared columns were tested for their enantioseparation efficiency of a range of racemic pharmaceuticals. The screening results demonstrated the potential of functionalizing polymer monolithic stationary phases with TM-β-CD using the in situ encapsulation approach.
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| http://dx.doi.org/10.1016/j.talanta.2016.06.027 | DOI Listing |
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