Selective enrichment of Eicosapentaenoic acid (20:5n-3) in N. oceanica CASA CC201 by natural auxin supplementation.

Bioresour Technol

Microbial Processes and Technology Division, National Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), Trivandrum, Kerala, India; Academy of Scientific and Innovative Research (AcSIR), CSIR, India. Electronic address:

Published: October 2017

AI Article Synopsis

  • The study investigates how different concentrations of the natural auxin Indole-3 acetic acid (IAA) affect the growth and lipid production in the microalgae Nannochloropsis oceanica CASA CC201.
  • A concentration of 10ppm IAA significantly increased the cell count to 579.5×10^6 cells/ml compared to 215.5×10^6 cells/ml in the control group.
  • At 40ppm IAA, the lipid accumulation and yield were highest at 60.9% DCW and 319.5 mg/L, respectively, also boosting the EPA content to 10.76% versus 1.87% in the control.

Article Abstract

The present study aims to evaluate the effect of different concentration of natural auxin, Indole-3 acetic acid (IAA) on growth, lipid yield, PUFA and EPA accumulation in Nannochloropsis oceanica CASA CC201. It was observed that the, treatment with 10ppm concentration of IAA resulted in high cell number 579.5×10cells/ml than the control (215.5×10cells/ml). Treatment with IAA at a concentration of 40ppm gives the highest cellular lipid accumulation of 60.9% DCW than the control 31.05% DCW). Lipid yield is also found to be increased by the addition of 40ppm IAA (319.5mg/L) compared with the control (121.5mg/L). EPA percentage is increased to 10.76% by the addition of 40ppm IAA compared to the control (1.87%).

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Source
http://dx.doi.org/10.1016/j.biortech.2017.03.149DOI Listing

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