AI Article Synopsis
- Cyclophilin D (HsCypD) was isolated from the freshwater pearl mussel, identified with a full-length cDNA of 2,671 bp that encodes a 367-amino-acid protein.
- HsCypD is characterized as a hydrophilic intracellular protein with 10 phosphorylation sites, four tetratricopeptide repeat (TPR) domains, and lacks a signal peptide.
- Phylogenetic analysis suggests a common ancestry for CypD across species, with HsCypD most closely related to that from the sea snail Lottia gigantea, and its mRNA expression shows tissue-specific patterns peaking in the intestines.
Article Abstract
Cyclophilin D (referred to as ) was obtained from the freshwater pearl mussel (). The full-length cDNA was 2 671 bp, encoding a protein consisting of 367 amino acids. HsCypD was determined to be a hydrophilic intracellular protein with 10 phosphorylation sites and four tetratricopeptide repeat (TPR) domains, but no signal peptide. The core sequence region YKGCIFHRIIKDFMVQGG is highly conserved in vertebrates and invertebrates. Phylogenetic tree analysis indicated that CypD from all species had a common origin, and HsCypD had the closest phylogenetic relationship with CypD from Lottia gigantea. The constitutive mRNA expression levels of exhibited tissue-specific patterns, with the highest level detected in the intestines, followed by the gonads, and the lowest expression found in the hemocytes.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5396027 | PMC |
| http://dx.doi.org/10.24272/j.issn.2095-8137.2017.018 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Environmental DNA Insights into the Spatial Status of Fish Diversity in the Mainstem of the Jialing River.
Animals (Basel)
January 2025
Laboratory of Water Ecological Health and Environmental Safety, School of Life Sciences, Chongqing Normal University, Chongqing 401331, China.
Preserving healthy river habitats is essential for maintaining fish diversity. Over time, anthropogenic activities have severely damaged river habitats, leading to notable changes in fish diversity patterns. Conducting thorough and reliable investigations into fish diversity is crucial for assessing anthropogenic impacts on diversity.
View Article and Find Full Text PDFOccurrence, Bioaccumulation, and Human Exposure Risk of the Antiandrogenic Fluorescent Dye 7-(Dimethylamino)-4-methylcoumarin and 7-(Diethylamino)-4-methylcoumarin in the Dongjiang River Basin, South China.
Toxics
December 2024
Key Laboratory of Ministry of Education for Water Quality Security and Protection in Pearl River Delta, School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China.
Recently, 7-diethylamino-4-methylcoumarin (DEAMC) has been identified as a potent antiandrogenic compound in the surface water; however, little is known about the antiandrogenic potentials of other synthetic coumarins and their occurrence in the aquatic ecosystem. In this study, for the first time, we observed that 7-dimethylamino-4-methylcoumarin (DAMC) elicited androgen receptor (AR) antagonistic activity with a 50% inhibitory concentration (IC) of 1.46 µM, which is 14.
View Article and Find Full Text PDFThe community dynamic alterations mechanisms of traveling plastics in the Pearl River estuary with the salinity influence.
Water Res
December 2024
College of Environment and Energy, South China University of Technology, Guangzhou 510006, China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006, China. Electronic address:
Most ocean plastics originate from terrestrial emissions, and the plastisphere on the plastics would alter during the traveling due to the significant differences in biological communities between freshwater and marine ecosystems. Microorganisms are influenced by the increasing salinity during traveling. To understand the contribution of plastic on the alteration in biological communities of plastisphere during traveling, this study investigated the alterations in microbial communities on plastics during the migration from freshwater to brackish water and saltwater.
View Article and Find Full Text PDFChromosome-level genome assembly of the ratmouth barbel, Ptychidio jordani.
Sci Data
December 2024
Key Laboratory of Prevention and Control for Aquatic Invasive Alien Species, Ministry of Agriculture and Rural Affairs, Guangdong Modern Recreational Fisheries Engineering Technology Center, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, China.
The ratmouth barbel (Ptychidio jordani) is a critically endangered freshwater fish from the Cyprinidae family, primarily due to overfishing and habitat disruption. To address the challenges of its shrinking wild populations and the difficulties in artificial reproduction, we sequenced, assembled, and annotated a high-quality chromosome-level genome of P. jordani using next-generation short-read sequencing, third-generation long-read sequencing, and Hi-C sequencing.
View Article and Find Full Text PDFDevelopment and Characterisation of an Immortal Cell Line From Largemouth Bass (Micropterus salmoides) for Viral Studies.
J Fish Dis
December 2024
Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
Article Synopsis
- Researchers successfully created an immortal cell line (MSF) from the fin tissue of Largemouth bass, which can be cultured continuously for over 80 passages.
- The MSF cells, maintained at 28°C and confirmed to be free from mycoplasma contamination, are identified as epithelialoid cells with specific chromosome characteristics.
- This new cell line is highly susceptible to various viruses affecting Largemouth bass, making it a valuable tool for future studies on gene expression and viral mechanics.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!