In Cellulo Approaches to Study Peroxisomal Protein Import - Yeast Immunofluorescence Microscopy.

Immunofluorescence microscopy is a powerful tool to analyze the localization of selected proteins in single cells. The technique allows the detection of endogenously expressed proteins as well as tags added to proteins of choice with specific antibodies. Originally evolved for human cell lines, protocols are now also available for yeast cells. Here, we describe an immunofluorescence microscopy technique for imaging peroxisomal matrix and membrane proteins of the yeast Saccharomyces cerevisiae.