Peroxisomes are present in virtually every eukaryotic cell type with the exception of the mature erythrocyte. In higher eukaryotes, one of the main functions of peroxisomes is lipid metabolism by means of beta-oxidation of very long-chain fatty acids (VLCFA; ≥22 carbon atoms). A dysfunction in peroxisomal VLCFA beta-oxidation results in elevated VLCFA levels in cells, tissue, and plasma. Here, we describe a straightforward and sensitive method to measure peroxisomal beta-oxidation capacity in living cells using stable-isotope labeled docosanoic acid (D-C22:0).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-4939-6937-1_5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Inhibition of CD36 ameliorates mouse spinal cord injury by accelerating microglial lipophagy.
Acta Pharmacol Sin
January 2025
Oujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, China.
Spinal cord injury (SCI) is a serious trauma of the central nervous system (CNS). SCI induces a unique lipid-dense environment that results in the deposition of large amounts of lipid droplets (LDs). The presence of LDs has been shown to contribute to the progression of other diseases.
View Article and Find Full Text PDFSynthetic mycolates derivatives to decipher protein mycoloylation, a unique post-translational modification in bacteria.
J Biol Chem
January 2025
Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198, Gif-sur-Yvette, France. Electronic address:
Protein mycoloylation is a newly characterized post-translational modification (PTM) specifically found in Corynebacteriales, an order of bacteria that includes numerous human pathogens. Their envelope is composed of a unique outer membrane, the so-called mycomembrane made of very-long chain fatty acids, named mycolic acids. Recently, some mycomembrane proteins including PorA have been unambiguously shown to be covalently modified with mycolic acids in the model organism Corynebacterium glutamicum by a mechanism that relies on the mycoloyltransferase MytC.
View Article and Find Full Text PDFSpermidine enhances the heat tolerance of by promoting mitochondrial respiration driven by fatty acid β-oxidation.
Appl Environ Microbiol
January 2025
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Unlabelled: High temperature is an unavoidable environmental stress that generally exerts detrimental effects on organisms and has widespread effects on metabolism. Spermidine is an important member of the polyamines family and is involved in a range of abiotic stress responses in plants. Mitochondria play an essential role in cellular homeostasis and are key components of the stress response.
View Article and Find Full Text PDFSaturated fat in an evolutionary context.
Lipids Health Dis
January 2025
Institute of Health, Oslo New University College, Ullevålsveien 76, Oslo, 0454, Norway.
Evolutionary perspectives have yielded profound insights in health and medical sciences. A fundamental recognition is that modern diet and lifestyle practices are mismatched with the human physiological constitution, shaped over eons in response to environmental selective pressures. This Darwinian angle can help illuminate and resolve issues in nutrition, including the contentious issue of fat consumption.
View Article and Find Full Text PDFFatty Acid Profiles Linked to Organohalogen Exposure in Cetaceans from the Northern South China Sea.
Environ Sci Technol
January 2025
School of Marine Sciences, Zhuhai Key Laboratory of Marine Bioresources and Environment, Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Pearl River Estuary Marine Ecosystem Research Station, Ministry of Education, Sun Yat-Sen University, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519082, China.
Increasing evidence suggests that organohalogen contaminants (OHCs) could disrupt lipid metabolism in organisms, prompting consideration of fatty acids (FAs) as biological tools for assessing chemical stress in biological systems. This study examined 87 OHCs and 32 FAs in two sentinel cetacean species─Indo-Pacific humpback dolphins ( = 128) and Indo-Pacific finless porpoises ( = 26)─from the northern South China Sea (NSCS), a global hotspot for OHCs. Our results revealed higher OHC levels in these cetaceans than global averages.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!