The next-generation sequencing (NGS) based RNA sequencing (RNA-Seq) and transcriptome profiling offers an opportunity to unveil complex biological processes. Successful RNA-Seq and transcriptome profiling requires a large amount of high-quality RNA. However, NGS-quality RNA isolation is extremely difficult from recalcitrant adipose tissue (AT) with high lipid content and low cell numbers. Further, the amount and biochemical composition of AT lipid varies depending upon the animal species which can pose different degree of resistance to RNA extraction. Currently available approaches may work effectively in one species but can be almost unproductive in another species. Herein, we report a two step protocol for the extraction of NGS quality RNA from AT across a broad range of animal species.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jcp.25951 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Proteomic characterization and comparison of the infective and adult life stage secretomes from Necator americanus and Ancylostoma ceylanicum.
PLoS Negl Trop Dis
January 2025
Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, Australia.
More than 470 million people globally are infected with the hookworms Ancylostoma ceylanicum and Necator americanus, resulting in an annual loss of 2.1 to 4 million disability-adjusted-life-years. Current infection management approaches are limited by modest drug efficacy, the costs associated with frequent mass drug administration campaigns, and the risk of reinfection and burgeoning drug resistance.
View Article and Find Full Text PDFAdvances in waste-derived functional materials for PFAS remediation.
Biodegradation
January 2025
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Rawamangun, Jakarta Timur, Indonesia.
Per- and polyfluoroalkyl substances (PFAS) are synthetic organofluoride compounds, widely used in industries since the 1950s for their hydrophobic properties. PFAS contamination of soil and water poses significant environmental and public health risks due to their persistence, chemical stability, and resistance to degradation. The Chemical Abstracts Service catalogs approximately 4300 PFAS globally.
View Article and Find Full Text PDFAvian haemosporidian parasites affecting non-descript village chickens in Africa.
Trop Anim Health Prod
January 2025
Department of Agriculture and Animal Health, College of Agriculture and Environmental Science, University of South Africa, Florida, South Africa.
Smallholder farmers in most of the rural areas in African countries rear non-descript village chickens for petty cash, food provision and for performing rituals. Village chicken production systems are regarded as low input- low output because the chickens receive minimum care and produce average to less eggs and meat. The chickens receive minimal biosecurity and are often left to scavenge for feed and thus exposes them to potential vector parasites that can transmit parasites such as haemoparasites.
View Article and Find Full Text PDFA female sterilization method for use in field-based behavioral studies of the invasive Asian longhorned beetle (Anoplophora glabripennis).
J Insect Sci
January 2025
Northern Research Station, U.S. Forest Service, United States Department of Agriculture, Hamden, CT, USA.
Asian longhorned beetle (Anoplophora glabripennis Motschulsky), a wood borer (Coleoptera: Cerambycidae) native to China, has been unintentionally and repeatedly introduced to North American and European landscapes as a stow-away in the wood packing material commonly used in international trade. Asian longhorned beetle causes extensive damage and mortality in multiple deciduous tree species and in response, countries in both North America and Europe have adopted policies of eradication. Models that integrate patterns of Asian longhorned beetle dispersal with records of infested trees are critical in optimizing survey and eradication efforts and tracking eradication progress.
View Article and Find Full Text PDF[Model selection for determination of primary stability of dental implants in experimental studies].
Stomatologiia (Mosk)
January 2025
Central Research Institute of Dental and Maxillofacial Surgery, Moscow, Russia.
Objective: Systematic review of literature on the pre-clinical studies of dental implantation in different models by finding out data about primary stability.
Materials And Methods: PubMed, NCBI, Wiley Online Library, MBPI, Elibrary systems were used for search.
Results: Based on the literature review of the described animal species dogs have the most similar bone structure to humans but their usage is complicated by ethics and law reasons.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!