Incorporation of synthetic degenerate oligonucleotides into plasmids for building highly diverse genetic libraries requires efficient and quantitative DNA manipulation. We present a fast and seamless method for generating libraries of PCR-synthesized plasmids designed with a degenerate sequence and short overlapping ends. Our method called QuickLib should find many applications in synthetic biology; as an example, we easily prepared genetic libraries of Escherichia coli expressing billions of different backbone cyclic peptides.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390991 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0175146 | PLOS |
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