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Strategy of employing plug-and-play vectors and LC-MS screening to facilitate the discovery of natural products using Aspergillus oryzae.
Bioresour Bioprocess
January 2025
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.
Aspergillus oryzae is a widely used host for heterologous expression of fungal natural products. However, the vectors previously developed are not convenient for use and screening positive transformants by PCR and fermentation is time- and effort-consuming. Hence, three plug-and-play vectors were developed here for multi-gene expression and liquid chromatography mass spectrometry detection was introduced to screen positive transformants.
View Article and Find Full Text PDFMix-Charged Nanofiltration Membrane for Efficient Organic Removal from High-Salinity Wastewater: The Role of Charge Spatial Distribution.
Environ Sci Technol
January 2025
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100190, PR China.
The efficient removal of organic contaminants from high-salinity wastewater is crucial for resource recovery and achieving zero discharge. Nanofiltration (NF) membranes are effective in separating organic compounds and monovalent salts, but they typically exhibit an excessive rejection of divalent salts. Modifying the charge characteristics of NF membranes can improve salt permeation; however, the role of charge spatial distribution in governing salt transport behavior is not fully understood.
View Article and Find Full Text PDFFirst Report of Postharvest Fruit Rot on Passion Fruit () caused by in the U.S.A.
Plant Dis
January 2025
University of Florida Tropical Research and Education Center, Plant Pathology, 1615 SE 23rd Way, Homestead, Florida, United States, 33031-3314;
The commercial production of passion fruit is geographically limited (California, Florida, and Hawaii), but the development of cold-tolerant varieties could expand it beyond warm-climate states (Stafne et.al. 2023).
View Article and Find Full Text PDFAn L-plug-and-play approach for MPI using a zero shot denoiser with evaluation on the 3D open MPI dataset.
Phys Med Biol
January 2025
Faculty of Mathematics and Natural Sciences , Hochschule Darmstadt, Schöfferstr., 3, Darmstadt, Hessen, 64295, GERMANY.
Article Synopsis
- Magnetic Particle Imaging (MPI) is a new medical imaging technique that provides high temporal resolution and avoids ionizing radiation by using the non-linear response of magnetic nanoparticles.
- A variety of regularization methods exist to reconstruct the particle concentration from the measured signals, including classical techniques and modern machine learning approaches, with this work focusing on a Plug-and-Play method using a zero-shot denoiser.
- The research validates the new reconstruction method using a hybrid dataset and compares its performance against established techniques, demonstrating its effectiveness and potential applicability in future MPI scenarios without the need for extensive training.
A fully integrated microfluidic cartridge for rapid and ultrasensitive nucleic acid detection from oropharyngeal swabs.
Lab Chip
January 2025
School of Biomedical Engineering, Tsinghua University, Haidian District, Beijing 100084, China.
Rapid and accurate molecular diagnostics are crucial for preventing the global spread of emerging infectious diseases. However, the current gold standard for nucleic acid detection, reverse transcription polymerase chain reaction (RT-PCR), relies heavily on traditional magnetic beads or silica membranes for nucleic acid extraction, resulting in several limitations, including time-consuming processes, the need for trained personnel, and complex equipment. Therefore, there is an urgent need for fully integrated nucleic acid detection technologies that are simple to operate, rapid, and highly sensitive to meet unmet clinical needs.
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