A combination strategy using two novel cerium-based nanocomposite affinity probes for the selective enrichment of mono- and multi-phosphopeptides in mass spectrometric analysis.

Chem Commun (Camb)

State Key Laboratory of Analytical Chemistry for Life Science, Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry & Chemical Engineering and Center of Materials Analysis, Nanjing University, 163 Xianlin Avenue, Nanjing 210023, P. R. China.

Published: April 2017

This work presents a sequential enrichment protocol, based on two self-designed cerium-based nanocomposite affinity probes, which not only can effectively separate phosphopeptides from non-phosphopeptides but can also selectively differentiate mono- and multi-phosphopeptides for direct matrix-assisted laser desorption/ionization time-of-flight mass spectrometric (MALDI-TOF MS) analysis.

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Source
http://dx.doi.org/10.1039/c7cc00540gDOI Listing

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