Food microbiologists are not yet able to monitor food and beverage production on-line with a device as simple and straight-forward as a pH meter. Most microbiological evaluations are limited by traditional labor intensive and time-consuming plating techniques. Test results are usually obtained after the product has left the manufacturer. The commercial development of biosensors for food analysis appears to be a long-term realization, however food microbiological and quality testing could benefit from the automation implicit in sensor technology. Current and future developments may include a miniature array of biosensors to rapidly perform, with high reproducibility, specificity, and sensitivity, various analytical techniques. Biosensors theoretically can be used to detect any compound of interest, be it a bacterial antigen, toxin, byproduct of microbial contamination, spoilage precursor, or additive adulterant. The following aspects of biosensors will be discussed: definitions and the evolution of various types of sensors; requirements for the food industry; currently available biosensors and their applications; advantages offered by and future uses of biosensors; and some problems in their commercial development.
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http://dx.doi.org/10.4315/0362-028X-51.10.811 | DOI Listing |
Nano Lett
January 2025
College of Life Science and Technology, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Huazhong University of Science and Technology, Wuhan 430074, China.
The pursuit of cutting-edge diagnostic systems capable of detecting biomarkers with exceptional sensitivity and precision is crucial for the timely and accurate monitoring of inflammatory responses. In this study, we introduce a dual gold nanoparticle-enhanced metasurface plasmon resonance (Bi-MSPR) biosensor for the ultrasensitive detection of C-reactive protein (CRP). The Bi-MSPR sensor is constructed upon a nanocup array chip with gradient-free electron density, where an innovative metasurface structure is built using a PEI-immobilized dual-gold nanoparticle amplification system.
View Article and Find Full Text PDFAnal Chem
January 2025
State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control of Jiangsu Province, Jiangnan University, Wuxi 214122, China.
Metal-organic framework-based hybrids (MOFzyme) have promising applications in colorimetric aptasensors due to their highly efficient and stable catalytic activity. However, their efficient application in biosensors remains a challenging issue due to the limited reaction site and amorphous structure. Herein, we encapsulated catalase inside MOF cavities to prepare an MOFzyme with many functional groups on its surface, and the functional groups were utilized for the subsequent integration of MOFzyme into the hyaluronic acid-DNA hydrogel.
View Article and Find Full Text PDFWorld J Clin Cases
January 2025
Department of Basic Sciences, Biomedical Stomatology Research Group, Universidad de Antioquia U de A, Medellín 0057, Colombia.
In a recent case report in the , emphasized the crucial role of rapidly and accurately identifying pathogens to optimize patient treatment outcomes. Laboratory-on-a-chip (LOC) technology has emerged as a transformative tool in health care, offering rapid, sensitive, and specific identification of microorganisms. This editorial provides a comprehensive overview of LOC technology, highlighting its principles, advantages, applications, challenges, and future directions.
View Article and Find Full Text PDFBiochemistry (Mosc)
December 2024
Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 119991, Russia.
Food safety is one of the primary demands of modern society. Mycotoxins are toxic metabolites of food-contaminating fungi. Fungi enter the food chain by infecting crops and irreversibly contaminate them due to the structural stability of mycotoxins.
View Article and Find Full Text PDFMikrochim Acta
January 2025
Department of Analytical Chemistry, Faculty of Pharmacy, "Iuliu Hațieganu" University of Medicine and Pharmacy, 4 Pasteur Street, 400349, Cluj-Napoca, Romania.
A label-free, flexible, and disposable aptasensor was designed for the rapid on-site detection of vancomycin (VAN) levels. The electrochemical sensor was based on lab-printed carbon electrodes (C-PE) enriched with cauliflower-shaped gold nanostructures (AuNSs), on which VAN-specific aptamers were immobilized as biorecognition elements and short-chain thiols as blocking agents. The AuNSs, characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM), enhanced the electrochemical properties of the platform and the aptamer immobilization active sites.
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