Root extractive from benefits in wound healing of anal fistula through up-regulation of collagen genes in human skin fibroblasts.

Wound healing is the main problem in the therapy of anal fistula (AF). root has been traditionally used as an agent to soak sutures in operation of AF patients, but its function in wound healing remains largely unclear. The aim of the present study was to illuminate mechanisms of root treatment on AF. In the present study, 60 AF patients after surgery were randomly divided into two groups, external applied with or without the extractive. Wound healing times were compared and granulation tissues were collected. , we constructed damaged human skin fibroblasts (HSFs) with the treatment of (10 μg/ml). Cell Count Kit-8 (CCK-8) and flow cytometry analysis were used to determine the effects of root extractive on cell viability, cell cycle and apoptosis of damaged HSFs. Furthermore, protein levels of , matrix metalloproteinase (MMP)-3 () and signalling pathways were investigated both and Results showed that root extractive greatly shortens the wound healing time in AF patients. In granulation tissues and HSFs, treatment with the extractive significantly elevated the expressions of and , while reduced the expression of Further detection presented that signalling was activated after the stimulation of extractive in HSFs. Our study demonstrated that extractive from root could effectively improve wound healing in patients with AF via the up-regulation of fibroblast proliferation and expressions of and .